EC Number | Application | Comment | Organism |
---|---|---|---|
1.13.11.39 | synthesis | a multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol | Paraburkholderia xenovorans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.39 | gene bphC, recombinant expression in Escherichia coli | Paraburkholderia xenovorans |
1.14.13.7 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.39 | biphenyl-2,3-diol + O2 | Paraburkholderia xenovorans | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.14.13.7 | phenol + NADPH + H+ + O2 | Pseudomonas sp. | - |
catechol + NADP+ + H2O | - |
? | |
1.14.13.7 | phenol + NADPH + H+ + O2 | Pseudomonas sp. OX1 | - |
catechol + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.39 | Paraburkholderia xenovorans | - |
- |
- |
1.14.13.7 | Pseudomonas sp. | - |
- |
- |
1.14.13.7 | Pseudomonas sp. OX1 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.39 | 3-methylcatechol + O2 | - |
Paraburkholderia xenovorans | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
1.13.11.39 | 4-methylcatechol + O2 | low activity | Paraburkholderia xenovorans | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | - |
Paraburkholderia xenovorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | additional information | multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol, substrate specificity and molecular docking, product identification by HPLC-MS, overview | Paraburkholderia xenovorans | ? | - |
? | |
1.14.13.7 | 3-cresol + NADPH + H+ + O2 | - |
Pseudomonas sp. | 3-methylcatechol + NADP+ + H2O | - |
? | |
1.14.13.7 | 3-cresol + NADPH + H+ + O2 | - |
Pseudomonas sp. OX1 | 3-methylcatechol + NADP+ + H2O | - |
? | |
1.14.13.7 | 4-cresol + NADPH + H+ + O2 | - |
Pseudomonas sp. | 4-methylcatechol + NADP+ + H2O | - |
? | |
1.14.13.7 | 4-cresol + NADPH + H+ + O2 | - |
Pseudomonas sp. OX1 | 4-methylcatechol + NADP+ + H2O | - |
? | |
1.14.13.7 | phenol + NADPH + H+ + O2 | - |
Pseudomonas sp. | catechol + NADP+ + H2O | - |
? | |
1.14.13.7 | phenol + NADPH + H+ + O2 | - |
Pseudomonas sp. OX1 | catechol + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.39 | 2,3-dihydroxy-biphenyl 1,2-dioxygenase | - |
Paraburkholderia xenovorans |
1.13.11.39 | BphCLA-4 | - |
Paraburkholderia xenovorans |
1.14.13.7 | phenol hydroxylase | - |
Pseudomonas sp. |
1.14.13.7 | PHIND | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 8 | - |
- |
Paraburkholderia xenovorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 5 | 10 | over 60% at pH 6.0 and 9.0, and less than 50% catalytic efficiency at pH 5.0 and 10.0, strain BphCLA-4 | Paraburkholderia xenovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.7 | NADPH | - |
Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.39 | additional information | enzyme structure homology modeling, overview | Paraburkholderia xenovorans |