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Literature summary extracted from

  • Wang, R.; Gu, X.; Yao, M.; Pan, C.; Liu, H.; Xiao, W.; Wang, Y.; Yuan, Y.
    Engineering of beta-carotene hydroxylase and ketolase for astaxanthin overproduction in Saccharomyces cerevisiae (2017), Front. Chem. Sci. Engin., 11, 89-99 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.99.63 synthesis construction of an astaxanthin biosynthesis pathway in Saccharomyces cerevisiae by introducing heterologous beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW) into an existing high beta-carotene producing strain. Astaxanthin yield of 3.1 mg/g dry cell weight are achieved. After change of promoter and hrough high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reaches to 81.0 mg/l Brevundimonas vesicularis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.B16 expressed in Saccharomyces cerevisiae strain Sc118030 Brevundimonas vesicularis
1.14.15.24 astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight Alcaligenes sp. PC-1
1.14.15.24 expressed in Saccharomyces cerevisiae Alcaligenes sp. PC-1
1.14.99.63 astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight Brevundimonas vesicularis
1.14.99.63 expression in Saccharomyces cerevisiae Brevundimonas vesicularis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.B16 beta-carotene + 2-oxoglutarate + O2 + H2O Brevundimonas vesicularis
-
 canthaxanthin + succinate + CO2 + 4 H+
-
 ?
1.14.11.B16 beta-carotene + 2-oxoglutarate + O2 + H2O Brevundimonas vesicularis DC263
-
 canthaxanthin + succinate + CO2 + 4 H+
-
 ?
1.14.15.24 beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Alcaligenes sp. PC-1
-
 beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Alcaligenes sp. PC-1 overall reaction zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Alcaligenes sp. PC-1
-
 zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.B16 Brevundimonas vesicularis
-
-
-
1.14.11.B16 Brevundimonas vesicularis DC263
-
-
-
1.14.15.24 Alcaligenes sp. PC-1
-
-
-
1.14.15.24 Alcaligenes sp. PC-1 Q44262
-
-
1.14.99.63 Brevundimonas vesicularis Q0H2C9
-
-
1.14.99.63 Brevundimonas vesicularis DC263 Q0H2C9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.B16 beta-carotene + 2-oxoglutarate + O2 + H2O
-
 Brevundimonas vesicularis canthaxanthin + succinate + CO2 + 4 H+
-
 ?
1.14.11.B16 beta-carotene + 2-oxoglutarate + O2 + H2O
-
 Brevundimonas vesicularis DC263 canthaxanthin + succinate + CO2 + 4 H+
-
 ?
1.14.15.24 beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Alcaligenes sp. PC-1 beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 overall reaction Alcaligenes sp. PC-1 zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Alcaligenes sp. PC-1 zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.B16 beta-carotene ketolase
-
 Brevundimonas vesicularis
1.14.11.B16 CrtW
-
 Brevundimonas vesicularis
1.14.15.24 beta-carotene hydroxylase
-
 Alcaligenes sp. PC-1
1.14.15.24 CrtZ
-
 Alcaligenes sp. PC-1
1.14.99.63 CrtW
-
 Brevundimonas vesicularis