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Literature summary extracted from

  • Olavarria, K.; Marone, M.P.; da Costa Oliveira, H.; Roncallo, J.C.; da Costa Vasconcelos, F.N.; da Silva, L.F.; Gomez, J.G.
    Quantifying NAD(P)H production in the upper Entner-Doudoroff pathway from Pseudomonas putida KT2440 (2015), FEBS open bio, 5, 908-915 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.363 gene zwf-1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and strain MG1655. The His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.363 NAD+
-
 Pseudomonas putida
1.1.1.363 NADH competitive inhibition Pseudomonas putida
1.1.1.363 NADP+ mixed-type inhibition Pseudomonas putida
1.1.1.363 NADPH inhibits competitively when NADP+ is the varying substrate and noncompetitively when D-glucose 6-phosphate is the varying substrate Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.363 additional information
-
 additional information kinetics and modelling, detailed overview Pseudomonas putida
1.1.1.363 0.014
-
 NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 0.127
-
 NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 0.946
-
 D-glucose 6-phosphate pH 8.0, 30°C, recombinant enzyme, with NADP+ Pseudomonas putida
1.1.1.363 1.137
-
 D-glucose 6-phosphate pH 8.0, 30°C, recombinant enzyme, with NAD+ Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.363 D-glucose 6-phosphate + NAD+ Pseudomonas putida
-
 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NAD+ Pseudomonas putida KT 2240
-
 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NADP+ Pseudomonas putida
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NADP+ Pseudomonas putida KT 2240
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.363 additional information Pseudomonas putida the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 ?
-
 ?
1.1.1.363 additional information Pseudomonas putida KT 2240 the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.363 Pseudomonas putida A0A1B2F298
-
-
1.1.1.363 Pseudomonas putida KT 2240 A0A1B2F298
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.363 D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ enzyme PputG6PDH-1 from Pseudomonas putida KT2440 catalyzes the oxidation of D-glucose 6-phosphate using a rapidequilibrium random mechanism Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.363 additional information
-
 the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida
1.1.1.363 0.006
-
 enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NADP+ Pseudomonas putida
1.1.1.363 0.008
-
 recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NAD+ Pseudomonas putida
1.1.1.363 0.025
-
 enzyme in Pseudomonas putida KT2440 crude cell extract, pH 8.0, 30°C, with NAD+ Pseudomonas putida
1.1.1.363 0.103
-
 recombinant enzyme in Escherichia coli MG1655 crude cell extract, pH 8.0, 30°C, with NADP+ Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.363 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 Pseudomonas putida D-glucose 6-phosphate + NAD+
-
 r
1.1.1.363 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 Pseudomonas putida KT 2240 D-glucose 6-phosphate + NAD+
-
 r
1.1.1.363 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 Pseudomonas putida D-glucose 6-phosphate + NADP+
-
 r
1.1.1.363 D-glucose 6-phosphate + NAD+
-
 Pseudomonas putida 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NAD+
-
 Pseudomonas putida KT 2240 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NADP+
-
 Pseudomonas putida 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.363 D-glucose 6-phosphate + NADP+
-
 Pseudomonas putida KT 2240 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.363 additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 Pseudomonas putida ?
-
 ?
1.1.1.363 additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme Pseudomonas putida ?
-
 ?
1.1.1.363 additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate, calculation of in vivo relative production of NADH and NADPH during the oxidation of glucose-6-phosphate. The reaction catalyzes by the 6-phosphogluconate dehydrogenase is active during growth on glucose, and it produces NADPH and NADH. In comparison, for the enzyme of Escherichia coli EcG6PDH, the relative production of NADH over NADPH is 1000times lower than for PputG6PDH-1 Pseudomonas putida KT 2240 ?
-
 ?
1.1.1.363 additional information the reaction catalyzed by PputG6PDH-1 yields around 1/3 mol of NADPH and 2/3 mol of NADH per mol of oxidized glucose 6-phosphate. PputG6PDH-1 is not considered a NADP+-specific enzyme Pseudomonas putida KT 2240 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.363 glucose-6-phosphate dehydrogenase
-
 Pseudomonas putida
1.1.1.363 PputG6PDH-1
-
 Pseudomonas putida
1.1.1.363 zwf-1
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.363 30
-
 assay at Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.363 102
-
 NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 105
-
 NADPH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 254
-
 NADH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 277
-
 NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.363 8
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.363 NAD+
-
 Pseudomonas putida
1.1.1.363 NADH
-
 Pseudomonas putida
1.1.1.363 NADP+ preferred cofactor Pseudomonas putida
1.1.1.363 NADPH
-
 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.363 0.035 0.163 NADPH pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 0.111
-
 NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 1.148
-
 NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.1.1.363 additional information the His-tagged form of PputG6PDH-1 shows no significant differences with respect to the non-tagged form of the same enzyme Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.363 2181
-
 NAD+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida
1.1.1.363 7286
-
 NADP+ pH 8.0, 30°C, recombinant enzyme Pseudomonas putida