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Literature summary extracted from

  • Ashraf, R.; Rashid, N.; Kanai, T.; Imanaka, T.; Akhtar, M.
    Pcal_1311, an alcohol dehydrogenase homologue from Pyrobaculum calidifontis, displays NADH-dependent high aldehyde reductase activity (2017), Extremophiles, 21, 1101-1110.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 Triton X-100 10% v/v, 3fold increase in activity Pyrobaculum calidifontis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 additional information presence of EDTA is not significantly inhibitory. Incubation with 100 mM EDTA at 70°C for 2 h results in loss of half of the enzyme activity. The enzyme is highly stable in the presence of up to 8 M urea or up to 6 M guanidine hydrochloride Pyrobaculum calidifontis
1.1.1.1 Sodium dodecyl sulfate 5 mM, complete loss of activity Pyrobaculum calidifontis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.072
-
 NADH pH 6, 60°C Pyrobaculum calidifontis
1.1.1.1 0.36
-
 1,4-Butanediol pH 10, 60°C Pyrobaculum calidifontis
1.1.1.1 0.67
-
 Glutaraldehyde pH 6, 60°C Pyrobaculum calidifontis
1.1.1.1 1.7
-
 NAD+ pH 10, 60°C Pyrobaculum calidifontis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn2+ Pcal_1311 is contains two zinc atoms per subunit. Twofold increase in enzyme activity of Pcal_1311 when produced in the presence of 25 microM Zn2+ as compared to the protein produced in tap water Pyrobaculum calidifontis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 76000
-
 gel filtration Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Pyrobaculum calidifontis A3MVR8
-
-
1.1.1.1 Pyrobaculum calidifontis JCM 11548 A3MVR8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1,2-butanediol + NAD+
-
 Pyrobaculum calidifontis ? + NADH + H+
-
 ?
1.1.1.1 1,4-butanediol + NAD+ best substrate in oxidation reaction Pyrobaculum calidifontis ? + NADH + H+
-
 ?
1.1.1.1 1-butanol + NAD+
-
 Pyrobaculum calidifontis butanal + NADH + H+
-
 ?
1.1.1.1 1-butanol + NAD+
-
 Pyrobaculum calidifontis JCM 11548 butanal + NADH + H+
-
 ?
1.1.1.1 1-hexanol + NAD+
-
 Pyrobaculum calidifontis hexanal + NADH + H+
-
 ?
1.1.1.1 1-hexanol + NAD+
-
 Pyrobaculum calidifontis JCM 11548 hexanal + NADH + H+
-
 ?
1.1.1.1 1-octanol + NAD+
-
 Pyrobaculum calidifontis octanal + NADH + H+
-
 ?
1.1.1.1 1-octanol + NAD+
-
 Pyrobaculum calidifontis JCM 11548 octanal + NADH + H+
-
 ?
1.1.1.1 1-pentanol + NAD+
-
 Pyrobaculum calidifontis pentanal + NADH + H+
-
 ?
1.1.1.1 acetaldehyde + NADH + H+ 5.7% of the activity with glutaraldehyde Pyrobaculum calidifontis ethanol + NAD+
-
 ?
1.1.1.1 acetaldehyde + NADH + H+ 5.7% of the activity with glutaraldehyde Pyrobaculum calidifontis JCM 11548 ethanol + NAD+
-
 ?
1.1.1.1 cinnamyl alcohol + NAD+
-
 Pyrobaculum calidifontis cinnamyl aldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+ 33% of the activity with 1,4-butanediol Pyrobaculum calidifontis acetaldehyde + NADH + H+
-
 ?
1.1.1.1 glutaraldehyde + NADH + H+ best substrate in reduction reaction Pyrobaculum calidifontis ? + NAD+
-
 ?
1.1.1.1 hexanal + NADH + H+ 69.6% of the activity with glutaraldehyde Pyrobaculum calidifontis 1-hexanol + NAD+
-
 ?
1.1.1.1 isobutanal + NADH + H+ 20% of the activity with glutaraldehyde Pyrobaculum calidifontis isobutanol + NAD+
-
 ?
1.1.1.1 mannitol + NAD+
-
 Pyrobaculum calidifontis ? + NADH + H+
-
 ?
1.1.1.1 additional information the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom Pyrobaculum calidifontis ?
-
 ?
1.1.1.1 additional information the enzyme catalyzes NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon atom Pyrobaculum calidifontis JCM 11548 ?
-
 ?
1.1.1.1 pentanal + NADH + H+ 38% of the activity with glutaraldehyde Pyrobaculum calidifontis 1-pentanol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 37555, calculated Pyrobaculum calidifontis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 alcohol dehydrogenase GroES domain protein
-
 Pyrobaculum calidifontis
1.1.1.1 Pcal_1311
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 80
-
-
 Pyrobaculum calidifontis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 80
-
 12 h, 80% residual activity Pyrobaculum calidifontis
1.1.1.1 90
-
 3 h, 90% residual activity. No significant change in the CD spectra up to 90 °C Pyrobaculum calidifontis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 1.98
-
 1,4-Butanediol pH 10, 60°C Pyrobaculum calidifontis
1.1.1.1 93.13
-
 Glutaraldehyde pH 6, 60°C Pyrobaculum calidifontis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
 sodium phosphate buffer, highest activity for reduction of acetaldehyde Pyrobaculum calidifontis
1.1.1.1 10
-
 glycine–NaOH buffer, highest activity for oxidation of ethanol Pyrobaculum calidifontis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 additional information no activity with NADP+ Pyrobaculum calidifontis
1.1.1.1 NAD+
-
 Pyrobaculum calidifontis
1.1.1.1 NADH
-
 Pyrobaculum calidifontis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.1 Pyrobaculum calidifontis
-
-
 6.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 5.5
-
 1,4-Butanediol pH 10, 60°C Pyrobaculum calidifontis
1.1.1.1 139
-
 Glutaraldehyde pH 6, 60°C Pyrobaculum calidifontis