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Literature summary extracted from

  • Zerva, A.; Christakopoulos, P.; Topakas, E.
    Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol (2015), Enzyme Microb. Technol., 75-76, 49-56 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.7 synthesis heterologous production in Pichia pastoris is greatlyenhanced by the addition of hemin with a titer of 0.41 U/ml peroxidase activity at the second day ofincubation Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 H2O2 enzyme retains more than 80% of its initial activity after 24 h incubation in 5000fold molar excess of H2O2 Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Thermothelomyces thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7
-
 Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 0.49
-
 substrate guaiacol, pH 5, 60°C Thermothelomyces thermophilus
1.11.1.7 0.55
-
 substrate 2,6-dimethyloxyphenol, pH 5, 60°C Thermothelomyces thermophilus
1.11.1.7 0.63
-
 substrate catechol, pH 5, 60°C Thermothelomyces thermophilus
1.11.1.7 2.46
-
 substrate pyrogallol, pH 5, 60°C Thermothelomyces thermophilus
1.11.1.7 4.13
-
 substrate hydroquinone, pH 5, 60°C Thermothelomyces thermophilus
1.11.1.7 178
-
 substrate 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid), pH 5, 60°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2 guaiacol + H2O2
-
 Thermothelomyces thermophilus 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
1.11.1.7 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Thermothelomyces thermophilus ?
-
 ?
1.11.1.7 2,6-dimethyloxyphenol + H2O2
-
 Thermothelomyces thermophilus ? + H2O
-
 ?
1.11.1.7 catechol + H2O2
-
 Thermothelomyces thermophilus ? + H2O
-
 ?
1.11.1.7 hydroquinone + H2O2
-
 Thermothelomyces thermophilus ? + H2O
-
 ?
1.11.1.7 additional information no substrates: veratryl alcohol, reactive black 5, Mn2+ Thermothelomyces thermophilus ?
-
 ?
1.11.1.7 pyrogallol + H2O2
-
 Thermothelomyces thermophilus ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 ? x * 50000, SDS-PAGE, x * 38500, calculated, x * 39000, SDS-PAGE of deglycosylated protein Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 PerII
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 60
-
 stable up to Thermothelomyces thermophilus