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Literature summary extracted from

  • Jaspers, M.H.; Pflanz, R.; Riedel, D.; Kawelke, S.; Feussner, I.; Schuh, R.
    The fatty acyl-CoA reductase Waterproof mediates airway clearance in Drosophila (2014), Dev. Biol., 385, 23-31 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.84 gene wat, recombinant expression in Saccharomyces cerevisiae strain H1246, recombinant expression of the enzyme and the peroxisomal marker mRFP-SKL in Drosophila S2 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.84 additional information construction of enzyme-defective mutants, phenotypes, overview Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.84 endoplasmic reticulum
-
 Drosophila melanogaster 5783
-
1.2.1.84 additional information no specific colocalisation of the enzyme with the peroxisomal marker SKL Drosophila melanogaster
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.84 hexacosanoyl-CoA + 2 NADPH + 2 H+ Drosophila melanogaster
-
 hexacosanoyl alcohol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 additional information Drosophila melanogaster Waterproof is a Drosophila FAR with a preference for very long chain, saturated fatty acids of 24 and 26 carbons. In yeast cultures expressing Waterproof, tetracosanoyl-alcohol (24:0-OH) and hexacosanoyl-alcohol (26:0-OH) are identified by GC/MS analysis, unsaturated alcohols or alcohols of shorter chain-lengths are either absent or below the detection limit. Neither of these fatty alcohols is detected in the vector control yeast culture or the wat gene containing culture grown under non-inducing conditions ?
-
 ?
1.2.1.84 tetracosanoyl-CoA + 2 NADPH + 2 H+ Drosophila melanogaster
-
 tetracosanoyl alcohol + 2 NADP+ + coenzyme A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.84 Drosophila melanogaster Q8MS59
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.84 embryo Waterproof reveals a restricted expression pattern during embryogenesis Drosophila melanogaster
-
1.2.1.84 additional information Waterproof acts in a non-cell-autonomous manner, overview Drosophila melanogaster
-
1.2.1.84 trachea
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.84 hexacosanoyl-CoA + 2 NADPH + 2 H+
-
 Drosophila melanogaster hexacosanoyl alcohol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 additional information Waterproof is a Drosophila FAR with a preference for very long chain, saturated fatty acids of 24 and 26 carbons. In yeast cultures expressing Waterproof, tetracosanoyl-alcohol (24:0-OH) and hexacosanoyl-alcohol (26:0-OH) are identified by GC/MS analysis, unsaturated alcohols or alcohols of shorter chain-lengths are either absent or below the detection limit. Neither of these fatty alcohols is detected in the vector control yeast culture or the wat gene containing culture grown under non-inducing conditions Drosophila melanogaster ?
-
 ?
1.2.1.84 tetracosanoyl-CoA + 2 NADPH + 2 H+
-
 Drosophila melanogaster tetracosanoyl alcohol + 2 NADP+ + coenzyme A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.84 CG1443 locus name Drosophila melanogaster
1.2.1.84 FAR
-
 Drosophila melanogaster
1.2.1.84 fatty acyl-CoA reductase
-
 Drosophila melanogaster
1.2.1.84 wat
-
 Drosophila melanogaster
1.2.1.84 Waterproof
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
1.2.1.84 malfunction in waterproof mutant embryos the formation of the outermost tracheal cuticle sublayer, the envelope is disrupted and the hydrophobic tracheal coating is damaged Drosophila melanogaster
1.2.1.84 physiological function Waterproof, encoding a fatty acyl-CoA reductase (FAR), is essential for the gas filling of the tracheal tubes during Drosophila embryogenesis, and does not affect branch network formation or key tracheal maturation processes. A non-cell-autonomous waterproof function for the beginning of the tracheal gas filling process, overview. Waterproof plays a key role in tracheal gas filling by providing very long chain fatty alcohols that serve as potential substrates for wax ester synthesis or related hydrophobic substances that ultimately coat the inner lining of the trachea. The hydrophobicity in turn reduces the tensile strength of the liquid inside the trachea, leading to the formation of a gas bubble, the focal point for subsequent gas filling. Enzyme activity is specifically required for the tracheal liquid clearance (LC). Waterproof is essential for outer envelope formation of the tracheal cuticle Drosophila melanogaster