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Literature summary extracted from
- Synthesis of 1-naphthol by a natural peroxygenase engineered by directed evolution (2016), ChemBioChem, 17, 341-349.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.2.1 | expressed in Saccharomyces cerevisiae | Cyclocybe aegerita |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.11.2.1 | F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L | this mutant retains strong activity and stability, particularly in terms of temperature and the presence of co-solvents, compared to the wild type enzyme | Cyclocybe aegerita |
| 1.11.2.1 | G241D/R257K | the mutant displays a 2fold improvement in peroxygenase activity and half the peroxidative activity of the wild type enzyme | Cyclocybe aegerita |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 0.048 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita |  |
| 1.11.2.1 | 0.126 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 0.127 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 0.181 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 0.486 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 0.578 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 0.866 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 1.25 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.11.2.1 | extracellular | - |
Cyclocybe aegerita | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.2.1 | Cyclocybe aegerita | B9W4V6 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.2.1 | 2 2,6-dimethoxyphenol + H2O2 | - |
Cyclocybe aegerita | cerulignone + 2 H2O | - |
? | |
| 1.11.2.1 | 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Cyclocybe aegerita | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
| 1.11.2.1 | naphthalene + H2O2 | - |
Cyclocybe aegerita | 1-naphthol + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.2.1 | ? | x * 52000, SDS-PAGE | Cyclocybe aegerita |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.2.1 | aromatic peroxygenase | - |
Cyclocybe aegerita |
| 1.11.2.1 | UPO1 | - |
Cyclocybe aegerita |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 68 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 78 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 125 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 142 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 229 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 238 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 395 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 447 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.2.1 | heme | - |
Cyclocybe aegerita | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.2.1 | 160 | - |
2,6-dimethoxyphenol | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 360 | - |
H2O2 | mutant enzyme G241D/R257K, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 400 | - |
Naphthalene | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 500 | - |
H2O2 | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, with 2,6-dimethoxyphenol as cosubstrate, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 540 | - |
2,6-dimethoxyphenol | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 620 | - |
Naphthalene | mutant enzyme G241D/R257K, at pH 7.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 690 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme G241D/R257K, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
| 1.11.2.1 | 8200 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme F12Y/A14V/R15GA21D/V57A/L67F/V75I/I248V/F311L, at pH 4.0, temperature not specified in the publication | Cyclocybe aegerita | |
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