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Literature summary extracted from

  • Wang, Z.; Sun, Y.; Shi, Y.; Song, W.; Zhang, C.
    Cloning, expression and characterization of a mesophilic catechol 1,2-dioxygenase from Rhodococcus ruber OA1 (2017), Biotechnology, 16, 10-18 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expressed in Escherichia coli BL21(DE3) cells Rhodococcus ruber

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 K+
-
 Rhodococcus ruber
1.13.11.1 Mg2+
-
 Rhodococcus ruber
1.13.11.1 NH4+
-
 Rhodococcus ruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Mn2+ Mn2+ enhances the activity of the enzyme Rhodococcus ruber

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Rhodococcus ruber
-
 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 Rhodococcus ruber OA1
-
 cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Rhodococcus ruber
-
-
-
1.13.11.1 Rhodococcus ruber OA1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 Ni-NTA resin column chromatography Rhodococcus ruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 catechol + O2
-
 Rhodococcus ruber cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Rhodococcus ruber OA1 cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 ? x * 31700, calculated from amino acid sequence Rhodococcus ruber

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C12O
-
 Rhodococcus ruber
1.13.11.1 CatA
-
 Rhodococcus ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 25
-
-
 Rhodococcus ruber

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.1 15 45 more than 50% activity between 15 and 45°C Rhodococcus ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7
-
-
 Rhodococcus ruber

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 4.5 8 more than 50% activity between pH 4.5 and 8.0 Rhodococcus ruber

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.1 Rhodococcus ruber calculated from amino acid sequence
-
 4.7