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Literature summary extracted from

  • Hwang, H.J.; Park, J.H.; Kim, J.H.; Kong, M.K.; Kim, J.W.; Park, J.W.; Cho, K.M.; Lee, P.C.
    Engineering of a butyraldehyde dehydrogenase of Clostridium saccharoperbutylacetonicum to fit an engineered 1,4-butanediol pathway in Escherichia coli (2014), Biotechnol. Bioeng., 111, 1374-1384 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.57 gene bld, butyraldehyde dehydrogenase gene of Clostridium saccharoperbutylacetonicum is coexpresses with diverse involved genes in Escherichia coli for the reconstruction of 1,4-butanediol biosynthesis pathway in Escherichia coli, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Clostridium saccharoperbutylacetonicum

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.57 A176T random mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 A467S random mutagenesis, the mutant shows similar activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 K279R random mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273C site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273I random mutagenesis, the mutant shows about 3.5fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273M site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273S site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273T site-directed mutagenesis, the mutant shows about 4fold increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 L273V site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 M371R random mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum
1.2.1.57 additional information to fit enzyme Bld into the non-natural 1,4-butanediol pathway, it is engineered through random mutagenesis, library construction. Five Bld mutants are isolated using a colorimetric Schiff's reagent-based method. Subsequent site-directed mutagenesis of Bld generates the two best Bld mutants, L273I and L273T, which produce 1,4-butanediol titers fourfold greater than those of wild-type Bld. Butyraldehyde dehydrogenase (Bld) and butanol dehydrogenase (Bdh) of Clostridium saccharoperbutylacetonicum are selected as a substitute for the bifunctional AdhE2 in the reconstructed 1,4-butanediol biosynthesis pathway in Escherichia coli, coexpression of diverse involved genes in Escherichia coli Clostridium saccharoperbutylacetonicum
1.2.1.57 N409T random mutagenesis, the mutant shows similar activity compared to the wild-type enzyme Clostridium saccharoperbutylacetonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.57 additional information
-
 additional information no Michaelis-Menten behaviour Clostridium saccharoperbutylacetonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.57 4-hydroxybutanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum
-
 4-hydroxybutanal + CoA + NAD+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
 4-hydroxybutanal + CoA + NAD+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum
-
 4-hydroxybutanal + CoA + NADP+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
 4-hydroxybutanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum
-
 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum
-
 butanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+ Clostridium saccharoperbutylacetonicum KCTC5577
-
 butanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.57 Clostridium saccharoperbutylacetonicum
-
-
-
1.2.1.57 Clostridium saccharoperbutylacetonicum KCTC5577
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.57 4-hydroxybutanoyl-CoA + NADH + H+
-
 Clostridium saccharoperbutylacetonicum 4-hydroxybutanal + CoA + NAD+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADH + H+
-
 Clostridium saccharoperbutylacetonicum KCTC5577 4-hydroxybutanal + CoA + NAD+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADPH + H+
-
 Clostridium saccharoperbutylacetonicum 4-hydroxybutanal + CoA + NADP+
-
 ?
1.2.1.57 4-hydroxybutanoyl-CoA + NADPH + H+
-
 Clostridium saccharoperbutylacetonicum KCTC5577 4-hydroxybutanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Clostridium saccharoperbutylacetonicum butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADH + H+
-
 Clostridium saccharoperbutylacetonicum KCTC5577 butanal + CoA + NAD+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+
-
 Clostridium saccharoperbutylacetonicum butanal + CoA + NADP+
-
 ?
1.2.1.57 butanoyl-CoA + NADPH + H+
-
 Clostridium saccharoperbutylacetonicum KCTC5577 butanal + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.57 Bld
-
 Clostridium saccharoperbutylacetonicum
1.2.1.57 Butyraldehyde dehydrogenase
-
 Clostridium saccharoperbutylacetonicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.57 NADH
-
 Clostridium saccharoperbutylacetonicum
1.2.1.57 NADPH
-
 Clostridium saccharoperbutylacetonicum

General Information

EC Number General Information Comment Organism
1.2.1.57 metabolism the enzyme is part of the 1,4-butanediol biosynthesis pathway, overview Clostridium saccharoperbutylacetonicum