EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.22 | degradation | enzyme completely hydrolyzes raffinose and stachyose present in soybeans and kidney beans at 50°C within 60 min | Rhizomucor miehei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expression in Escherichia coli | Rhizomucor miehei |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.22 | enzyme displays remarkable resistance to proteases | Rhizomucor miehei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | 4% of initial activity | Rhizomucor miehei | |
3.2.1.22 | Hg2+ | 8% of initial activity | Rhizomucor miehei | |
3.2.1.22 | additional information | not inhibitory: D-galactose at 100 mM, or dithiothreitol, EDTA | Rhizomucor miehei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.36 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 16.9 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 27.6 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 47.9 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ca2+ | 129% of initial activity | Rhizomucor miehei | |
3.2.1.22 | Co2+ | 195% of initial activity | Rhizomucor miehei | |
3.2.1.22 | Cu2+ | 119% of initial activity | Rhizomucor miehei | |
3.2.1.22 | Fe2+ | 194% of initial activity | Rhizomucor miehei | |
3.2.1.22 | Zn2+ | 116% of initial activity | Rhizomucor miehei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 312000 | - |
gel filtration | Rhizomucor miehei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Rhizomucor miehei | H8Y263 | - |
- |
3.2.1.22 | Rhizomucor miehei CAU432 | H8Y263 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 198 | - |
substrate 4-nitrophenyl alpha-D-galactopyranoside, pH 4.5, 50°C | Rhizomucor miehei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizomucor miehei CAU432 | 4-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | melibiose + H2O | 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | alpha-D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | alpha-D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | raffinose + H2O | 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | D-galactose + sucrose | - |
? | |
3.2.1.22 | raffinose + H2O | 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + H2O | 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | raffinose + D-galactose | - |
? | |
3.2.1.22 | stachyose + H2O | 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | raffinose + D-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.22 | tetramer | 4 * 85141, calculated, 4 * 85000, SDS-PAGE | Rhizomucor miehei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | Gal36 | - |
Rhizomucor miehei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 60 | - |
- |
Rhizomucor miehei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 55 | - |
30 min, 100% residual activity | Rhizomucor miehei |
3.2.1.22 | 60 | - |
30 min, 70% reidual activity | Rhizomucor miehei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.65 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 4.2 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 6.3 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 6.4 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.5 | - |
- |
Rhizomucor miehei |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.5 | 10 | - |
Rhizomucor miehei |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.22 | Rhizomucor miehei | isoelectric focusing | - |
5.8 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.04 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 0.09 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 0.23 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei | |
3.2.1.22 | 17.5 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei |