Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Choi, S.K.; Lin, M.T.; Ouyang, H.; Gennis, R.B.
    Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli (2017), Biochim. Biophys. Acta, 1858, 366-370 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.3 expressed in Escherichia coli C43 BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.3 D36V the mutant of subunit III shows 160% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 E164A the mutant of subunit I shows 28% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 E164K the mutant of subunit I shows 54% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 E259A the mutant of subunit II shows 135% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 E259K the mutant of subunit II shows 97% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 F165A the mutant of subunit I shows 37% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 F29I the mutant of subunit III shows 115% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 K25L the mutant of subunit III shows75 % activity compared to the wild type enzyme Escherichia coli
7.1.1.3 L171A the mutant of subunit I shows 79% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 Q167A the mutant of subunit I shows 57% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 Q167K the mutant of subunit I shows 75% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 Q195L the mutant of subunit I shows 119% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 R176A the mutant of subunit III shows 70% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 S177A the mutant of subunit I shows 91% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 T168A the mutant of subunit I shows 118% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 T247V the mutant of subunit I shows 173% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 W136A the mutant of subunit II shows 89% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 W136K the mutant of subunit II shows 105% activity compared to the wild type enzyme Escherichia coli
7.1.1.3 W156A the mutant of subunit III shows 70% activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.3 0.015
-
ubiquinol-1 mutant enzyme D36V, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.02
-
ubiquinol-1 mutant enzyme E164A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.021
-
ubiquinol-1 mutant enzyme T247V, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.026
-
ubiquinol-1 mutant enzyme Q167K, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.027
-
ubiquinol-1 mutant enzyme E259A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.027
-
ubiquinol-1 mutant enzyme E259K, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.027
-
ubiquinol-1 mutant enzyme Q167A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.031
-
ubiquinol-1 mutant enzyme T168A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.032
-
ubiquinol-1 mutant enzyme S177A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.033
-
ubiquinol-1 mutant enzyme F165A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.033
-
ubiquinol-1 mutant enzyme W136A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.039
-
ubiquinol-1 mutant enzyme W136K, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.042
-
ubiquinol-1 mutant enzyme L171A, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.046
-
ubiquinol-1 mutant enzyme E164K, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.05
-
ubiquinol-1 wild type enzyme, at pH 7.0 and 25°C Escherichia coli
7.1.1.3 0.067
-
ubiquinol-1 mutant enzyme Q195L, at pH 7.0 and 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.3 Cu2+ contains copper Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 ubiquinol-1 + O2 + H+[side 1]
-
Escherichia coli ubiquinone-1 + H2O + H+[side 2]
-
?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 cytochrome bo3 oxidase
-
Escherichia coli
7.1.1.3 cytochrome bo3 ubiquinol oxidase
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.3 heme
-
Escherichia coli