Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yenugudhati, D.; Prakash, D.; Kumar, A.K.; Kumar, R.S.; Yennawar, N.H.; Yennawar, H.P.; Ferry, J.G.
    Structural and biochemical characterizations of methanoredoxin from Methanosarcina acetivorans, a glutaredoxin-like enzyme with coenzyme M-dependent protein disulfide reductase activity (2016), Biochemistry, 55, 313-321 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.8
-
 Methanosarcina acetivorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.8 crystal structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. The crystal lattice has four monomers in a dimer of dimers arrangement. A cadmium ion is found within the active site of each monomer, and two cadmium ions stabilize the N-terminal tails and dimer interfaces. CoMSH and glutathione bind to the active site of methanoredoxin similar to the binding of glutathione in glutaredoxin Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.8 2.2
-
 coenzyme M pH 6.8, 50°C Methanosarcina acetivorans
1.8.1.8 4.1
-
 coenzyme A pH 6.8, 50°C Methanosarcina acetivorans
1.8.1.8 8.9
-
 glutathione disulfide pH 6.8, 50°C Methanosarcina acetivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.8 12000
-
 gel filtration Methanosarcina acetivorans

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.8 Methanosarcina acetivorans Q8TQ93
-
-
1.8.1.8 Methanosarcina acetivorans DSM 2834 Q8TQ93
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.8 coenzyme M disulfide + 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Methanosarcina acetivorans 2 coenzyme M + thionitrobenzoate
-
 ?
1.8.1.8 coenzyme M disulfide + 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Methanosarcina acetivorans DSM 2834 2 coenzyme M + thionitrobenzoate
-
 ?
1.8.1.8 coenzyme M disulfide + glutathione disulfide
-
 Methanosarcina acetivorans 2 coenzyme M + glutathione
-
 ?
1.8.1.8 coenzyme M disulfide + glutathione disulfide
-
 Methanosarcina acetivorans DSM 2834 2 coenzyme M + glutathione
-
 ?
1.8.1.8 insulin disulfide + 2 coenzyme A
-
 Methanosarcina acetivorans insulin + coenzyme A disulfide
-
 ?
1.8.1.8 insulin disulfide + 2 coenzyme A
-
 Methanosarcina acetivorans DSM 2834 insulin + coenzyme A disulfide
-
 ?
1.8.1.8 insulin disulfide + 2 coenzyme M
-
 Methanosarcina acetivorans insulin + coenzyme M disulfide
-
 ?
1.8.1.8 insulin disulfide + 2 coenzyme M
-
 Methanosarcina acetivorans DSM 2834 insulin + coenzyme M disulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.8 monomer 1 * 11500, calculated and SDS-PAGE Methanosarcina acetivorans

Synonyms

EC Number Synonyms Comment Organism
1.8.1.8 glutaredoxin
-
 Methanosarcina acetivorans
1.8.1.8 MA_1658
-
 Methanosarcina acetivorans
1.8.1.8 methanoredoxin
-
 Methanosarcina acetivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.8 coenzyme M
-
 Methanosarcina acetivorans
1.8.1.8 additional information no cofactor: coenzyme B Methanosarcina acetivorans