Literature summary extracted from

Rivard, B.S.; Rogers, M.S.; Marell, D.J.; Neibergall, M.B.; Chakrabarty, S.; Cramer, C.J.; Lipscomb, J.D.
Rate-determining attack on substrate precedes rieske cluster oxidation during cis-dihydroxylation by benzoate dioxygenase (2015), Biochemistry, 54, 4652-4664 .

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.12.10	benzoate + NADH + H+ + O2	Pseudomonas putida	-	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+	-	?
1.14.12.10	benzoate + NADH + H+ + O2	Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182	-	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.12.10	Pseudomonas putida	-	-	-
1.14.12.10	Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.12.10	benzoate + NADH + H+ + O2	-	Pseudomonas putida	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+	-	?
1.14.12.10	benzoate + NADH + H+ + O2	-	Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.12.10	benzoate 1,2-dioxygenase oxygenase	-	Pseudomonas putida
1.14.12.10	BZDO	-	Pseudomonas putida

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.14.12.10	4.4	-	benzoate	at pH 6.8 temperature not specified in the publication	Pseudomonas putida

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.12.10	NADH	-	Pseudomonas putida
1.14.12.10	[2Fe-2S]-center	-	Pseudomonas putida

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Release 2023.1 (January 2023)