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Literature summary extracted from

  • Kudalkar, S.N.; Njuma, O.J.; Li, Y.; Muldowney, M.; Fuanta, N.R.; Goodwin, D.C.
    A role for catalase-peroxidase large loop 2 revealed by deletion mutagenesis control of active site water and ferric enzyme reactivity (2015), Biochemistry, 54, 1648-1662 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 E291A the mutant shows 89% peroxidase activity and 139% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 E291K the mutant shows 200% peroxidase activity and 79% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 E291K/E292A the mutant shows 222% peroxidase activity and 102% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 I290A the mutant shows 31% peroxidase activity and 93% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 I290A/Q293A the mutant shows 56% peroxidase activity and 64% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 I290V the mutant shows 35% peroxidase activity and 67% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 I290V/Q293V the mutant shows 44% peroxidase activity and 47% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 Q293A the mutant shows 59% peroxidase activity and 102% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 Q293E the mutant shows 62% peroxidase activity and 80% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
1.11.1.21 Q293V the mutant shows 25% peroxidase activity and 44% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.023
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.028
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.037
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.05
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.06
-
H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.06
-
H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.07
-
H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.071
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.078
-
H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.08
-
H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.08
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.081
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.087
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.089
-
H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.09
-
H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.09
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.1
-
H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.13
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.16
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.21
-
H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.31
-
H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 0.5
-
H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 13.3
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 13.6
-
H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 15.2
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 20
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 22.5
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 23.8
-
H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 24
-
H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 26
-
H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 26
-
H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 26
-
H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 27.8
-
H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 28.5
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 29.3
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 29.6
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 32.5
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 34
-
H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 35.3
-
H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 38
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 39.7
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 40
-
H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 55
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 83
-
H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 49
-
H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 110
-
H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 140
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 160
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 200
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 210
-
H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 220
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 268
-
H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 270
-
H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 280
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 310
-
H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 350
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 350
-
H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 370
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 380
-
H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 390
-
H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 390
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 560
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 600
-
H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 620
-
H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 630
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1.11.1.21 1260
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis