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Literature summary extracted from

  • Gwenin, V.; Poornima, P.; Halliwell, J.; Ball, P.; Robinson, G.; Gwenin, C.
    Identification of novel nitroreductases from Bacillus cereus and their interaction with the CB1954 prodrug (2015), Biochem. Pharmacol., 98, 392-402 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.38 gene BC_1619, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.38 0.2
-
NADPH with FMN, pH 7.2, 37°C Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.38 60000
-
recombinant His-tagged enzyme, gel filtration Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.38 Bacillus cereus Q81BW8
-
-
1.5.1.38 Bacillus cereus ATCC 14579 Q81BW8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.38 recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and desalting gel filtration Bacillus cereus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.1.38 insolubleoverexpressed enzyme protein is resuspended in a resuspension buffer containing 20 ml, 20 mM Tris-HCL, 0.5 M NaCl, pH 8.0, sonicated for 50 s discontinuously, spun down at 8000 rpm (5400 x g) at 4°C for 10 min, pellets resuspended in isolation buffer containing 15 ml, 20 mM Tris-HCL, 0.5 M NaCl, 2% Triton X-100, and 12% w/v urea, pH 8.0 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.38 FMNH2 + NADP+ FMN is enzyme-bound Bacillus cereus FMN + NADPH + H+
-
r
1.5.1.38 FMNH2 + NADP+ FMN is enzyme-bound Bacillus cereus ATCC 14579 FMN + NADPH + H+
-
r
1.5.1.38 additional information the nitroreductase of Bacillus cereus strain ATCC 14579 encoded by gene BC_1619 also shows NADPH-dependent FMN reductase activity, and interacts with the prodrug 5-(1-aziridinyl)-2,4-dinitrobenzamide, i.e. CB1954, converting it to either the toxic 2'- or 4'-hydroxylamine metabolites with cofactor NAD(P)H, overview Bacillus cereus ?
-
?
1.5.1.38 additional information the nitroreductase of Bacillus cereus strain ATCC 14579 encoded by gene BC_1619 also shows NADPH-dependent FMN reductase activity, and interacts with the prodrug 5-(1-aziridinyl)-2,4-dinitrobenzamide, i.e. CB1954, converting it to either the toxic 2'- or 4'-hydroxylamine metabolites with cofactor NAD(P)H, overview Bacillus cereus ATCC 14579 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.38 homodimer 2 * 26000-34000, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.5.1.38 BC_1619
-
Bacillus cereus
1.5.1.38 More cf. EC 1.7.12 Bacillus cereus
1.5.1.38 ydgI
-
Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.38 37
-
assay at Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.38 1.5
-
NADPH with FMN, pH 7.2, 37°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.38 7.2
-
assay at Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.38 NADP+
-
Bacillus cereus
1.5.1.38 NADPH
-
Bacillus cereus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.38 7.3
-
NADPH with FMN, pH 7.2, 37°C Bacillus cereus