EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.38 | gene BC_1619, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha | Bacillus cereus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.38 | 0.2 | - |
NADPH | with FMN, pH 7.2, 37°C | Bacillus cereus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.38 | 60000 | - |
recombinant His-tagged enzyme, gel filtration | Bacillus cereus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.38 | Bacillus cereus | Q81BW8 | - |
- |
1.5.1.38 | Bacillus cereus ATCC 14579 | Q81BW8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.38 | recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and desalting gel filtration | Bacillus cereus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.5.1.38 | insolubleoverexpressed enzyme protein is resuspended in a resuspension buffer containing 20 ml, 20 mM Tris-HCL, 0.5 M NaCl, pH 8.0, sonicated for 50 s discontinuously, spun down at 8000 rpm (5400 x g) at 4°C for 10 min, pellets resuspended in isolation buffer containing 15 ml, 20 mM Tris-HCL, 0.5 M NaCl, 2% Triton X-100, and 12% w/v urea, pH 8.0 | Bacillus cereus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.38 | FMNH2 + NADP+ | FMN is enzyme-bound | Bacillus cereus | FMN + NADPH + H+ | - |
r | |
1.5.1.38 | FMNH2 + NADP+ | FMN is enzyme-bound | Bacillus cereus ATCC 14579 | FMN + NADPH + H+ | - |
r | |
1.5.1.38 | additional information | the nitroreductase of Bacillus cereus strain ATCC 14579 encoded by gene BC_1619 also shows NADPH-dependent FMN reductase activity, and interacts with the prodrug 5-(1-aziridinyl)-2,4-dinitrobenzamide, i.e. CB1954, converting it to either the toxic 2'- or 4'-hydroxylamine metabolites with cofactor NAD(P)H, overview | Bacillus cereus | ? | - |
? | |
1.5.1.38 | additional information | the nitroreductase of Bacillus cereus strain ATCC 14579 encoded by gene BC_1619 also shows NADPH-dependent FMN reductase activity, and interacts with the prodrug 5-(1-aziridinyl)-2,4-dinitrobenzamide, i.e. CB1954, converting it to either the toxic 2'- or 4'-hydroxylamine metabolites with cofactor NAD(P)H, overview | Bacillus cereus ATCC 14579 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.38 | homodimer | 2 * 26000-34000, SDS-PAGE | Bacillus cereus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.38 | BC_1619 | - |
Bacillus cereus |
1.5.1.38 | More | cf. EC 1.7.12 | Bacillus cereus |
1.5.1.38 | ydgI | - |
Bacillus cereus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.38 | 37 | - |
assay at | Bacillus cereus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.38 | 1.5 | - |
NADPH | with FMN, pH 7.2, 37°C | Bacillus cereus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.38 | 7.2 | - |
assay at | Bacillus cereus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.38 | NADP+ | - |
Bacillus cereus | |
1.5.1.38 | NADPH | - |
Bacillus cereus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.38 | 7.3 | - |
NADPH | with FMN, pH 7.2, 37°C | Bacillus cereus |