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Literature summary extracted from

  • Hwang, J.; Espenshade, P.J.
    Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2 (2016), Biochem. J., 473, 2463-2469 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.11.1.11 analysis APEX2, i.e. engineered mutant K14D/W41F/E112K/A134P of APX, fused to a protein of interest covalently tags nearby proteins with biotin-phenol when H2O2 is added. High osmolarity and disruption of cell wall integrity permits live-cell biotin labeling in Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. APEX2 permits targeted and proximity-dependent labeling of proteins Glycine max

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11
-
 Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.11 K14D/W41F/E112K/A134P APEX2, i.e. engineered mutant of APX. APEX2 fused to a protein of interest covalently tags nearby proteins with biotin-phenol when H2O2 is added. High osmolarity and disruption of cell wall integrity permits live-cell biotin labeling in Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. APEX2 permits targeted and proximity-dependent labeling of proteins Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Glycine max Q76LA6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 commercial preparation
-
 Glycine max
-

Subunits

EC Number Subunits Comment Organism
1.11.1.11 ? x * 28000, SDS-PAGE, recombinant protein Glycine max

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 APEX2
-
 Glycine max