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Literature summary extracted from

  • Liu, X.; Yuan, Z.; Wang, J.; Cui, Y.; Liu, S.; Ma, Y.; Gu, L.; Xu, S.
    Crystal structure and biochemical features of dye-decolorizing peroxidase YfeX from Escherichia coli O157 Asp(143) and Arg(232) play divergent roles toward different substrates (2017), Biochem. Biophys. Res. Commun., 484, 40-44 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.19 in complex with heme, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 20% (w/v) PEG3350 and 0.1 M Bis-Tris pH 5.5 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.19 D143A the mutant shows no activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and guaiacol, but about 20% of wild type activity with Reactive Blue 19 and 10% activity with catechol Escherichia coli
1.11.1.19 D143N the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
1.11.1.19 H215A the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
1.11.1.19 L246W the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
1.11.1.19 R232E the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 40% of wild type activity with guaiacol and 35% activity with catechol Escherichia coli
1.11.1.19 R232L the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 90% of wild type activity with guaiacol and 50% activity with catechol Escherichia coli
1.11.1.19 S234Y the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Escherichia coli
-
-
-
1.11.1.19 Escherichia coli O157
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 Ni-chelating affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli O157 ?
-
 ?
1.11.1.19 catechol + H2O2 + H+
-
 Escherichia coli ?
-
 ?
1.11.1.19 catechol + H2O2 + H+
-
 Escherichia coli O157 ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Escherichia coli ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+
-
 Escherichia coli O157 ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2
-
 Escherichia coli ?
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2
-
 Escherichia coli O157 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Escherichia coli
1.11.1.19 YfeX
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 4 5
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Escherichia coli