Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Uemura, T.; Kita, A.; Watanabe, Y.; Adachi, M.; Kuroki, R.; Morimoto, Y.
    The catalytic mechanism of decarboxylative hydroxylation of salicylate hydroxylase revealed by crystal structure analysis at 2.5 A resolution (2016), Biochem. Biophys. Res. Commun., 469, 158-163 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.1 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.1 complexed with FAD, sitting drop vapor diffusion method, using 1.26 M ammonium sulfate, 200 mM lithium sulfate and 100 mM Tris-HCl buffer at pH 8.5 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2 Pseudomonas putida S-1
-
 catechol + NAD+ + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas putida
-
-
-
1.14.13.1 Pseudomonas putida S-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 Ni-NTA column chromatography and Superdex 75 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas putida S-1 catechol + NAD+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 ? x * 54000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 SALH
-
 Pseudomonas putida
1.14.13.1 salicylate hydroxylase
-
 Pseudomonas putida
1.14.13.1 salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating)
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD
-
 Pseudomonas putida
1.14.13.1 NADH
-
 Pseudomonas putida