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Literature summary extracted from

  • Zhang, Y.; Weiner, J.
    Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans sulfide quinone oxidoreductase (SQR) (2014), Arch. Biochem. Biophys., 564, 110-119 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.4 expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.4 C128A the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 C128S the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 C160A the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 C160S the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 C356A inactive Acidithiobacillus ferrooxidans
1.8.5.4 C356S the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 H132A the mutant shows strongly reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.5.4 H198A the mutant shows severely reduced activity compared to the wild type enzyme Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.4 0.00297
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.00343
-
 decylubiquinone wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.00425
-
 decylubiquinone mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.005
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.0054
-
 decylubiquinone mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.00553
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.0056
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.00585
-
 decylubiquinone mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.4 Acidithiobacillus ferrooxidans B7JBP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.4 nickel affinity column chromatography Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.4 sulfide + decylubiquinone
-
 Acidithiobacillus ferrooxidans sulfur + decylubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.4 ? x * 49000, SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
1.8.5.4 SQR
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.4 70
-
-
 Acidithiobacillus ferrooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.4
-
 45 the recombinant enzyme is highly stable when incubated at 0°C, 4°C, 23°C or 30°C with more than 90% of the activity remaining after 7 h. It is less stable at 37°C and loses about 40% of the activity in the first 7.5 h. The activity of the enzyme decreases more rapidly at 45°C with a half-life of approximately 5 h Acidithiobacillus ferrooxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.5.4 0.1
-
 Sulfide mutant enzyme C160A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.1
-
 Sulfide mutant enzyme C356S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.3
-
 Sulfide mutant enzyme C198A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 0.5
-
 Sulfide mutant enzyme C160S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 1
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 1.4
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 1.6
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 6.5
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.4 6.5 7
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.4 FAD
-
 Acidithiobacillus ferrooxidans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.5.4 180
-
 Sulfide mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 240
-
 decylubiquinone mutant enzyme C128A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 250
-
 decylubiquinone mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 270
-
 decylubiquinone mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 270
-
 Sulfide mutant enzyme H132A, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 290
-
 Sulfide mutant enzyme C128S, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 1890
-
 decylubiquinone wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans
1.8.5.4 2190
-
 Sulfide wild type enzyme, at pH 7.0 and 23°C Acidithiobacillus ferrooxidans