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Literature summary extracted from

  • Rados, D.; Turner, D.L.; Catarino, T.; Hoffart, E.; Neves, A.R.; Eikmanns, B.J.; Blombach, B.; Santos, H.
    Stereospecificity of Corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol (2016), Appl. Microbiol. Biotechnol., 100, 10573-10583 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene butACg, subcloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with the genes encoding ALS and ALDC of Lactobacillus lactis strain MG1363 Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B20 moe construction and engineering of Corynebacterium glutamicum strain DELTAaceEDELTApqoDELTAldhA(pEKEx2-als,aldB,butACg). Chromosomal inactivation of the putative BDH gene butACg (cg2958) in strain DELTAaceEDELTApqoDELTAldhA. BDH activity is nearly abolished upon inactivation of butACg indicating that Corynebacterium glutamicum expresses a single BDH under the experimental conditions examined. BDH activity increases 3fold in strain DELTAaceEDELTApqoDELTAldhA(pEKEx2-als,aldB,butACg) compared to the respective control. The inactivation of butACg gene decreases the BDH activity 75fold for the DELTAaceEDELTApqoDELTAldhADELTAbutACg(pEKEx2) strain compared to strain DELTAaceEDELTApqoDELTAldhA(pEKEx2). The major form of 2,3-butanediol is meso-2,3-butandediol, and the ratio meso-2,3-BD/optically active 2,3-BD is 95:5, the main side products are glycerol, ethanol, and acetoin Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B20 (2S,3S)-2,3-butanediol product inhibition Corynebacterium glutamicum
1.1.1.B20 additional information no inhibition by NAD+ at 2 mM and 5 mM Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B20 0.052
-
(2S,3S)-2,3-butanediol pH 10.5, 30°C, recombinant enzyme Corynebacterium glutamicum
1.1.1.B20 0.23
-
acetoin racemic (R/S)-acetoin, pH 7.0, 30°C, recombinant enzyme Corynebacterium glutamicum
1.1.1.B20 1.49
-
(R)-acetoin pH 7.0, 30°C, recombinant enzyme Corynebacterium glutamicum
1.1.1.B20 2.01
-
meso-2,3-butanediol pH 10.5, 30°C, recombinant enzyme Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B20 0.3
-
recombinant Escherichia coli strain BL21(DE3) expressing wild-type BDH, pH 7.0, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (R)-acetoin + NADH + H+
-
Corynebacterium glutamicum meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 (S)-acetoin + NADH + H+
-
Corynebacterium glutamicum meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 2 acetoin + 2 NADH + 2 H+ racemic mixture of (3S/3R)-acetoin Corynebacterium glutamicum (2S,3S)-2,3-butanediol + meso-2,3-butanediol + 2 NAD+
-
r
1.1.1.B20 additional information NMR identification and quantification of reaction products. Meso-2,3-butanediol is the major form (over 95% of the 2,3-butanediol pool, depending on oxygen availability) produced in fermentations using a strain that overexpresses ALS/ALDC of Lactobacillus lactis and BDH of Corynebacterium glutamicum, i.e., strain DELTAaceEDELTApqoDELTAldhA(pEKEx2als,aldB,butACg) Corynebacterium glutamicum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 2,3-butanediol dehydrogenase
-
Corynebacterium glutamicum
1.1.1.B20 BDH
-
Corynebacterium glutamicum
1.1.1.B20 butACg
-
Corynebacterium glutamicum
1.1.1.B20 meso-2,3-BDH
-
Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B20 30
-
assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B20 7
-
acetoin reduction assay at Corynebacterium glutamicum
1.1.1.B20 10.5
-
2,3-butanediol oxidation assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
Corynebacterium glutamicum
1.1.1.B20 NADH
-
Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.1.1.B20 evolution enzyme BDH belongs to the SDR family, of enzymes Corynebacterium glutamicum
1.1.1.B20 metabolism pathways for the synthesis of 2,3-butanediol in bacteria, overview Corynebacterium glutamicum