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Literature summary extracted from

  • Molina-Espeja, P.; Garcia-Ruiz, E.; Gonzalez-Perez, D.; Ullrich, R.; Hofrichter, M.; Alcalde, M.
    Directed evolution of unspecific peroxygenase from Agrocybe aegerita (2014), Appl. Environ. Microbiol., 80, 3496-3507 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.2.1 expressed in Saccharomyces cerevisiae Cyclocybe aegerita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.025
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita
1.11.2.1 0.684
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
1.11.2.1 1.37
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 1.9
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 5.2
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita B9W4V6
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.2.1 glycoprotein
-
 Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 12-(4-nitrophenoxy)dodecanoic acid + H2O2
-
 Cyclocybe aegerita ?
-
 ?
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Cyclocybe aegerita oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.2.1 2,6-dimethoxyphenol + H2O2
-
 Cyclocybe aegerita ?
-
 ?
1.11.2.1 5-nitro-1,3-benzodioxole + H2O2
-
 Cyclocybe aegerita 4-nitrocatechol + formic acid + H2O
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
1.11.2.1 veratryl alcohol + H2O2
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.2.1 ? x * 35942, calculated from amino acid sequence Cyclocybe aegerita
1.11.2.1 ? x * 46000, SDS-PAGE Cyclocybe aegerita

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 APO
-
 Cyclocybe aegerita
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita
1.11.2.1 UPO1
-
 Cyclocybe aegerita

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.2.1 53
-
 the enzyme shows 50% activity after 1 h at 53°C Cyclocybe aegerita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 88
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 219
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
1.11.2.1 221
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita
1.11.2.1 290
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 329
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 4.4
-
 optimum pH for 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Cyclocybe aegerita
1.11.2.1 6.5
-
 optimum pH for 5-nitro-1,3-benzodioxole Cyclocybe aegerita
1.11.2.1 7
-
 optimum pH for 2,6-dimethoxyphenol Cyclocybe aegerita

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.1 heme
-
 Cyclocybe aegerita

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 17
-
 veratryl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 174
-
 benzyl alcohol at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 211
-
 H2O2 at pH 7.0 and 20°C Cyclocybe aegerita
1.11.2.1 320
-
 5-nitro-1,3-benzodioxole at pH 6.5 and 20°C Cyclocybe aegerita
1.11.2.1 8800
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 4.4 and 20°C Cyclocybe aegerita