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Literature summary extracted from

  • Huang, L.; Liu, Y.; Sun, Y.; Yan, Q.; Jiang, Z.
    Biochemical characterization of a novel L-asparaginase with low glutaminase activity from Rhizomucor miehei and its application in food safety and leukemia treatment (2014), Appl. Environ. Microbiol., 80, 1561-1569 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.1 food industry the final level of acrylamide in biscuits and bread is decreased by about 81.6% and 94.2%, respectively, upon treatment with 10 U asnase per mg flour Rhizomucor miehei
3.5.1.1 medicine enzyme potentiates a lectin's induction of leukemic K562 cell apoptosis, allowing lowering of the drug dosage and shortening of the incubation time Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-mercaptoethanol 55% residual activity Rhizomucor miehei
3.5.1.1 Ag+ 2 mM, 42% residual activity Rhizomucor miehei
3.5.1.1 Ba2+ 2 mM, 30% residual activity Rhizomucor miehei
3.5.1.1 Co2+ 2 mM, 69% residual activity Rhizomucor miehei
3.5.1.1 Cr2+ 2 mM, 37% residual activity Rhizomucor miehei
3.5.1.1 Cu2+ 2 mM, 13% residual activity Rhizomucor miehei
3.5.1.1 EDTA 24% residual activity Rhizomucor miehei
3.5.1.1 Fe3+ 2 mM, 41% residual activity Rhizomucor miehei
3.5.1.1 Hg2+ 2 mM, 24% residual activity Rhizomucor miehei
3.5.1.1 Mg2+ 2 mM, 28% residual activity Rhizomucor miehei
3.5.1.1 SDS 56% residual activity Rhizomucor miehei
3.5.1.1 Zn2+ 2 mM, 17% residual activity Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.133
-
 L-asparagine pH 7.0, 45°C Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 133500
-
 gel filtration Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Rhizomucor miehei W0G253
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 1985
-
 pH 7.0, 45°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Rhizomucor miehei L-aspartate + NH3
-
 ?
3.5.1.1 additional information enzyme shows negligible activity with both D-asparagine (22 U/mg) and L-glutamine (36 U/mg) as the substrates Rhizomucor miehei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer 2 * 75316, calculated, 2 * 72000, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASNase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 45
-
-
 Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 45
-
 stable for 30 min Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 0.025
-
 L-asparagine pH 7.0, 45°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7
-
-
 Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 4 8 more than 80% of maxiumum activity Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Rhizomucor miehei calculated
-
 6.8