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Literature summary extracted from
- Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (2013), Appl. Environ. Microbiol., 79, 113-120 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | additional information | cofactors might be required for the sulfate-dependent enzyme activation dissociated from the enzyme complex during purification | Acidithiobacillus ferrooxidans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.99.B2 | recombinant enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Acidithiobacillus ferrooxidans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | Na2SO4 | 150 mM Na-sulfate is required to generate maximum activity, 60% inhibition at 200 mM | Acidithiobacillus ferrooxidans |  |
| 1.8.99.B2 | sulfite | activates 1.6fold at 2 mM, higher concentration of sulfite ions results in inhibition of the enzyme, and almost complete inhibition at 10 mM sulfite | Acidithiobacillus ferrooxidans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | Na2SO4 | 150 mM Na-sulfate is required to generate maximum activity, 60% inhibition at 200 mM | Acidithiobacillus ferrooxidans | |
| 1.8.99.B2 | sulfite | activates 1.6fold at 2 mM, higher concentration of sulfite ions results in inhibition of the enzyme, and almost complete inhibition at 10 mM sulfite | Acidithiobacillus ferrooxidans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 25000 | - |
gel filtration | Acidithiobacillus ferrooxidans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.B2 | Acidithiobacillus ferrooxidans | B7J3E3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.B2 | native periplasmic enzyme from strain ATCC 23270 and recombinant enzyme from Escherichia coli strain BL21(DE3) celll extract by ammonium sulfate fractionation, cation exchange chromatography, dialysis, hydrophobic interaction chromatography, ultrafilatration, and gel filtration | Acidithiobacillus ferrooxidans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.99.B2 | cell culture | enzyme activity measured using ferricyanide as the electron acceptor is detected in cell extracts of Astrain ATCC 23270 grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells | Acidithiobacillus ferrooxidans | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 6.5 | - |
pH 2.5, 50°C, with ferricyanide | Acidithiobacillus ferrooxidans |
| 1.8.99.B2 | 13.8 | - |
pH 2.5, 70°C, with ferricyanide | Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.B2 | 2 thiosulfate + ferricyanide | - |
Acidithiobacillus ferrooxidans | tetrathionate + ferrocyanide | - |
? | |
| 1.8.99.B2 | additional information | nonenzymatic reduction of ferricyanide is observed with thiosulfate | Acidithiobacillus ferrooxidans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | monomer | 1 * 25000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | AFE_0042 | locus name | Acidithiobacillus ferrooxidans |
| 1.8.99.B2 | tetrathionate-forming thiosulfate dehydrogenase | - |
Acidithiobacillus ferrooxidans |
| 1.8.99.B2 | tetrathionate-forming TSD | - |
Acidithiobacillus ferrooxidans |
| 1.8.99.B2 | TSD | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 70 | - |
- |
Acidithiobacillus ferrooxidans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 2.5 | - |
- |
Acidithiobacillus ferrooxidans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | ferricyanide | - |
Acidithiobacillus ferrooxidans | |
| 1.8.99.B2 | additional information | no heme is detected. Ubiquinone and cytochrome C are no cofactors | Acidithiobacillus ferrooxidans |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | physiological function | thiosulfate dehydrogenase plays a significant role in thiosulfate oxidation in the acidophilic, obligately chemolithoautotroph, Acidithiobacillus ferrooxidans | Acidithiobacillus ferrooxidans |
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