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Literature summary extracted from

  • Wu, Z.; Hou, Y.; Dai, Z.; Hu, C.A.; Wu, G.
    Metabolism, nutrition, and redox signaling of hydroxyproline (2017), Antioxid. Redox Signal., 30, 674-682.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.B1 to 2.7 A resolution, space group of P43212, one physiological homodimer per asymmetric unit. Structure displays distinct binding sites for thioredoxin and the glutaredoxin domain, a single glutathione disulfide reduction site in the Grx domain, and rotation of the glutaredoxin domain toward the Sec-containing redox active site Echinococcus granulosus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.B1 C519S about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid) Echinococcus granulosus
1.8.1.B1 C574S about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid) Echinococcus granulosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 [mu-[1-(hydroxy-1kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]-[mu-[1-(hydroxy-2kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]bis(triphenylphosphane)digold a single gold atom binds to Cys519 and Cys573 in the AuI-enzyme complex. 75–80% inhibition of thioredoxin and glutathione reductase activities is achieved at a 1:1 concentration ratio of inhibitor:enzyme Echinococcus granulosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.012
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.013
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.015
-
 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.033
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.034
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.038
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.5.3 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.5.3 trans-4-hydroxy-L-proline + a quinone Homo sapiens
-
 (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + a quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.3 Homo sapiens
-
-
-
1.8.1.B1 Echinococcus granulosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.3 trans-4-hydroxy-L-proline + a quinone
-
 Homo sapiens (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + a quinol
-
 ?
1.8.1.B1 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Echinococcus granulosus 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.B1 glutathione disulfide + NADPH + H+
-
 Echinococcus granulosus glutathione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.5.3 hydroxyproline oxidase
-
 Homo sapiens
1.5.5.3 OH-POX
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 2 8 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 5
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 5.6
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 10.2
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 12
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 55
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.B1 FAD
-
 Echinococcus granulosus
1.8.1.B1 NADPH
-
 Echinococcus granulosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.B1 0.31
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.35
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.38
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 0.47
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 1.45
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.8.1.B1 1.87
-
 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus