EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.365 | gene gaaA, expression analysis in wild-type strain and in a strain overexpressing the D-galacturonic acid-specific GatA transporter | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.365 | D-galacturonate + NADPH + H+ | Aspergillus niger | - |
L-galactonate + NADP+ | - |
r | |
1.1.1.365 | D-galacturonate + NADPH + H+ | Aspergillus niger CBS 120.49 | - |
L-galactonate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.365 | Aspergillus niger | A8DRH9 | - |
- |
1.1.1.365 | Aspergillus niger CBS 120.49 | A8DRH9 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.365 | mycelium | - |
Aspergillus niger | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger | L-galactonate + NADP+ | - |
r | |
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.365 | gaaA | - |
Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.365 | 7 | - |
assay at | Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.365 | NADP+ | - |
Aspergillus niger | |
1.1.1.365 | NADPH | - |
Aspergillus niger |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.365 | metabolism | after import via the D-galacturonic acid transporter encoded by An14g04280, D-galacturonic acid is catabolized by three key enzymes: D-galacturonic acid reductase, L-galactonate dehydratase, and 2-keto-3-deoxy-galactonate aldolase, in Aspegillus niger. The first step in the D-galacturonic acid metabolism is the enzymatic conversion of D-galacturonic acid to L-galactonic acid by D-galacturonic acid reductase. Overexpression of the Aspergillus niger GatA transporter leads to preferential uptake of D-galacturonic acid over D-xylose of mutant strain JS013 and enanced use of D-galacturonic acid compared to D-xylose. Increased activity of the D-galacturonic acid metabolic pathway is observed for strain JS013 transformant strain in comparison to the control | Aspergillus niger |