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Literature summary extracted from
- Highly thermostable xylanase purified from Rhizomucor miehei NRL 3169 (2011), Acta Biol. Hung., 62, 85-94 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.8 | Ag2+ | 5 mM, 20% residual activtiy | Rhizomucor miehei |  |
| 3.2.1.8 | dithiothreitol | 5 mM, 70% residual activtiy | Rhizomucor miehei | |
| 3.2.1.8 | Hg2+ | 5 mM, 10% residual activtiy | Rhizomucor miehei | |
| 3.2.1.8 | Zn2+ | 5 mM, 52% residual activtiy | Rhizomucor miehei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.8 | extracellular | - |
Rhizomucor miehei | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.8 | Ca2+ | 5 mM, 126% of initial activity | Rhizomucor miehei | |
| 3.2.1.8 | Cu2+ | 5 mM, 116% of initial activity | Rhizomucor miehei | |
| 3.2.1.8 | K+ | 5 mM, 145% of initial activity | Rhizomucor miehei | |
| 3.2.1.8 | Na+ | 5 mM, 112% of initial activity | Rhizomucor miehei | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.8 | Rhizomucor miehei | - |
- |
- |
| 3.2.1.8 | Rhizomucor miehei NRL 3169 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.8 | - |
Rhizomucor miehei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 1849 | - |
pH 5.0, 50°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.8 | birchwood xylan + H2O | - |
Rhizomucor miehei | ? | - |
? | |
| 3.2.1.8 | birchwood xylan + H2O | - |
Rhizomucor miehei NRL 3169 | ? | - |
? | |
| 3.2.1.8 | additional information | no substrates: carboxymethyl cellulose, cellulose, cellobiose | Rhizomucor miehei | ? | - |
? | |
| 3.2.1.8 | additional information | no substrates: carboxymethyl cellulose, cellulose, cellobiose | Rhizomucor miehei NRL 3169 | ? | - |
? | |
| 3.2.1.8 | oat spelt xylan + H2O | - |
Rhizomucor miehei | ? | - |
? | |
| 3.2.1.8 | oat spelt xylan + H2O | - |
Rhizomucor miehei NRL 3169 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | ? | x * 27000, SDS-PAGE | Rhizomucor miehei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 75 | - |
- |
Rhizomucor miehei |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 90 | - |
half-life 30 min | Rhizomucor miehei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 5 | 6.5 | - |
Rhizomucor miehei |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | additional information | - |
birchwood xylan | Km value 0.055 mg/ml, pH 5.0, 50°C | Rhizomucor miehei |
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