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Literature summary extracted from

  • Hupert-Kocurek, K.; Wojcieszynska, D.; Guzik, U.
    Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp. strain S5 by random mutagenesis (2014), Acta Biochim. Pol., 61, 705-710 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli BL21 cells Planococcus sp. S5

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 A23T/F212S the mutant shows 40.65% activity with catechol compared to the wild type enzyme Planococcus sp. S5
1.13.11.2 F191I/C268R/Y272H/V280A/Y293D the mutant shows 38.29% activity with catechol compared to the wild type enzyme Planococcus sp. S5
1.13.11.2 H24R/F168S/Q275R the mutant shows 19.66% activity with catechol compared to the wild type enzyme Planococcus sp. S5
1.13.11.2 R296Q the mutant shows 128.47% activity with catechol compared to the wild type enzyme. The mutation also improves the activity of the enzyme against 4-chlorocatechol (582.04% activity compared to the wild type enzyme) Planococcus sp. S5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Planococcus sp. S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Planococcus sp. S5 E7DDG2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 0.00073
-
 mutant enzyme A23T/F212S, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.0009
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00108
-
 mutant enzyme H24R/F168S/Q275R, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00138
-
 mutant enzyme R296Q, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.0018
-
 wild type enzyme, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00272
-
 mutant enzyme A23T/F212S, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00298
-
 mutant enzyme H24R/F168S/Q275R, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00547
-
 mutant enzyme H24R/F168S/Q275R, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00572
-
 mutant enzyme H24R/F168S/Q275R, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00806
-
 mutant enzyme A23T/F212S, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00811
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00827
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00878
-
 mutant enzyme A23T/F212S, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.00963
-
 wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.01487
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.01966
-
 wild type enzyme, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.02062
-
 wild type enzyme, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.02223
-
 mutant enzyme R296Q, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.02775
-
 mutant enzyme R296Q, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
1.13.11.2 0.05605
-
 mutant enzyme R296Q, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Planococcus sp. S5 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2
-
 Planococcus sp. S5 4-chloro-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Planococcus sp. S5 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C23o
-
 Planococcus sp. S5