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Literature summary extracted from

  • Tuck, L.R.; Altenbach, K.; Ang, T.F.; Crawshaw, A.D.; Campopiano, D.J.; Clarke, D.J.; Marles-Wright, J.
    Insight into coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans (2016), Sci. Rep., 6, 22108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Lachnoclostridium phytofermentans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.3 in complex with NAD+, sitting drop vapor diffusion method, using 0.1 M sodium acetate pH 4.7, 1.8 M ammonium sulfate Lachnoclostridium phytofermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Butyraldehyde
-
 Lachnoclostridium phytofermentans
1.2.1.3 hexanaldehyde
-
 Lachnoclostridium phytofermentans
1.2.1.3 pentanaldehyde
-
 Lachnoclostridium phytofermentans
1.2.1.3 propionaldehyde
-
 Lachnoclostridium phytofermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.82
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 1.74
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 2.2
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 2.9
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 5.16
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 191300
-
 native mass spectrometry Lachnoclostridium phytofermentans

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Lachnoclostridium phytofermentans A9KN57
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 HisTrap column chromatography, Q-sepharose column chromatography, and S200 gel filtration Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans acetate + NADH + H+
-
 ?
1.2.1.3 butyraldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans butyrate + NADH + H+
-
 ?
1.2.1.3 hexanaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans hexanoate + NADH + H+
-
 ?
1.2.1.3 pentanaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans pentanoate + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O
-
 Lachnoclostridium phytofermentans propanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homotetramer x-ray crystallography Lachnoclostridium phytofermentans

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDDH
-
 Lachnoclostridium phytofermentans
1.2.1.3 Cphy_1178 locus name Lachnoclostridium phytofermentans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 1.62
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 3.44
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 3.45
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 5.44
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 5.61
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Lachnoclostridium phytofermentans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.3 12.61
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 17.31
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 23.52
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 144.3
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.3 0.31
-
 acetaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 1.93
-
 hexanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 1.98
-
 Butyraldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 2.47
-
 pentanaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans
1.2.1.3 4.21
-
 propionaldehyde at pH 8.0 and 21°C Lachnoclostridium phytofermentans