EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.16 | structures of apoenzyme and in complex with NAD+, to 1.85 and 1.9 A resolution. Two domain protein with the active site located in the interdomain interface. The NAD+ molecule is bound in the long channel with its nicotinamide ring positioned close to the side chain of the catalytic Cys268 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.16 | D426A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | E442A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | F138A | decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | K160A | Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | K160E | Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | K92A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | N193A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | R145A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | R428A | activity similar to wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | S425A | decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
1.2.1.16 | W141A | strong decrease in activity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | Succinic semialdehyde | substrate inhibition above 0.1 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 0.022 | - |
succinate semialdehyde | mutant R428A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.027 | - |
succinate semialdehyde | wild-type, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.043 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.047 | - |
succinate semialdehyde | wild-type, cofactor NADP+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.12 | - |
succinate semialdehyde | mutant F138A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.14 | - |
succinate semialdehyde | mutant E442A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.15 | - |
NAD+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.22 | - |
succinate semialdehyde | mutant S425A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.23 | - |
succinate semialdehyde | mutant D426A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.48 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.77 | - |
succinate semialdehyde | mutant W141A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2.4 | - |
NADP+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2.49 | - |
succinate semialdehyde | mutant R145A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2.6 | - |
succinate semialdehyde | mutant K92A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 10 | - |
NAD+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 11 | - |
NAD+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 26 | - |
NADP+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 27 | - |
NADP+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.16 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZPI3 | - |
- |
1.2.1.16 | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | Q8ZPI3 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | succinate semialdehyde + NAD+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | succinate + NADH + 2 H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NAD+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | succinate + NADH + 2 H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | succinate + NADPH + 2 H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | succinate + NADPH + 2 H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.16 | YneI | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 0.3 | - |
succinate semialdehyde | mutant D426A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2 | 8 | succinate semialdehyde | mutant R428A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2.1 | - |
NADP+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2.6 | - |
NADP+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 14 | - |
NAD+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 21 | - |
NADP+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 26 | - |
succinate semialdehyde | mutant F138A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 38 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 39 | - |
NAD+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 45 | - |
succinate semialdehyde | mutant R145A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 46 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 55 | - |
succinate semialdehyde | wild-type, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 66 | - |
succinate semialdehyde | mutant W141A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 68 | - |
succinate semialdehyde | mutant S425A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 73 | - |
succinate semialdehyde | mutant K92A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 80 | - |
NAD+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 83 | - |
succinate semialdehyde | wild-type, cofactor NADP+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 164 | - |
succinate semialdehyde | mutant E442A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | NAD+ | affinity to NAD+ is 10 times higher than to NADP+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | NADP+ | affinity to NAD+ is 10 times higher than to NADP+ | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 0.1 | - |
NADP+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 0.77 | - |
NADP+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 1.3 | - |
succinate semialdehyde | mutant D426A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 3.8 | - |
NAD+ | mutant K160A , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 7.3 | - |
NAD+ | mutant K160E , pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 8.6 | - |
NADP+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 18 | - |
succinate semialdehyde | mutant R145A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 28 | - |
succinate semialdehyde | mutant K92A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 86 | - |
succinate semialdehyde | mutant W141A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 95 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 97 | - |
NAD+ | mutant N193A, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 210 | - |
succinate semialdehyde | mutant F138A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 310 | - |
succinate semialdehyde | mutant S425A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 880 | - |
NAD+ | wild-type, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 1100 | - |
succinate semialdehyde | mutant E442A, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 1300 | - |
succinate semialdehyde | mutant R428A , cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 1800 | - |
succinate semialdehyde | wild-type, cofactor NADP+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.2.1.16 | 2000 | - |
succinate semialdehyde | wild-type, cofactor NAD+, pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |