Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jiang, L.; Gao, Z.; Li, Y.; Wang, S.; Dong, Y.
    Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus (2014), Protein Sci., 23, 366-377.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expressed in Escherichia coli BL21(DE3) cells Candidatus Liberibacter asiaticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 apoform and in complex with NAD+, hanging drop vapor diffusion method, using Candidatus Liberibacter asiaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 isoniazid competitive inhibitor with respect to NADH Candidatus Liberibacter asiaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.0175
-
 crotonyl-CoA at pH 7.5 and 28°C Candidatus Liberibacter asiaticus
1.3.1.9 0.0205
-
 NADH at pH 7.5 and 28°C Candidatus Liberibacter asiaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 61600
-
 gel filtration Candidatus Liberibacter asiaticus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Candidatus Liberibacter asiaticus C6XEY3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 Ni2+ Hi-Trap column chromatography and Superdex 200 gel filtration Candidatus Liberibacter asiaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Candidatus Liberibacter asiaticus butyryl-CoA + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 hexamer x-ray crystallography Candidatus Liberibacter asiaticus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-acyl carrier protein reductase I
-
 Candidatus Liberibacter asiaticus
1.3.1.9 ENR
-
 Candidatus Liberibacter asiaticus
1.3.1.9 FabI
-
 Candidatus Liberibacter asiaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Candidatus Liberibacter asiaticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.9 0.00011
-
 isoniazid with respect to NADH, at pH 7.5 and 28°C Candidatus Liberibacter asiaticus
1.3.1.9 0.0068
-
 isoniazid with respect to crotonyl-CoA, at pH 7.5 and 28°C Candidatus Liberibacter asiaticus