BRENDA - Enzyme Database

Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars

Zhang, X.; Carter, M.; Vetting, M.; Francisco, B.; Zhao, S.; Al-Obaidi, N.; Solbiati, J.; Thiaville, J.; Crecy-Lagard, V.; Jacobson, M.; Almo, S.; Gerlt, J.; Proc. Natl. Acad. Sci. USA 113, E4161-E4169 (2016)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.408
0.054
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
0.12
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
0.19
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
0.2
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
0.59
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
2.4
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.13
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
0.3
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.35
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.97
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.408
Cupriavidus necator
Q0K4F5
-
-
1.1.1.408
Cupriavidus necator DSM 428
Q0K4F5
-
-
1.1.1.408
Salmonella enterica subsp. enterica serovar Typhimurium
P58718
-
-
1.1.1.408
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
P58718
-
-
1.1.1.409
Cupriavidus necator
Q0K4F5
the enzyme additionally catalyzes the reaction of EC 1.1.1.408
-
1.1.1.409
Cupriavidus necator DSM 428
Q0K4F5
the enzyme additionally catalyzes the reaction of EC 1.1.1.408
-
1.1.1.409
Salmonella enterica subsp. enterica serovar Typhimurium
P58718
the enzyme additionally catalyzes the reaction of EC 1.1.1.408
-
1.1.1.409
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
P58718
the enzyme additionally catalyzes the reaction of EC 1.1.1.408
-
1.1.1.410
Cupriavidus necator
Q0KBD2
-
-
1.1.1.410
Cupriavidus necator DSM 428
Q0KBD2
-
-
1.1.1.411
Cupriavidus necator
Q0KBC7
-
-
1.1.1.411
Cupriavidus necator DSM 428
Q0KBC7
-
-
1.1.1.411
Pectobacterium atrosepticum
Q6CZ26
-
-
1.1.1.411
Pectobacterium atrosepticum ATCC BAA-672
Q6CZ26
-
-
2.7.1.217
Cupriavidus necator
Q0KBC8
-
-
2.7.1.217
Cupriavidus necator DSM 428
Q0KBC8
-
-
2.7.1.217
Salmonella enterica subsp. enterica serovar Typhimurium
Q8ZMG5
-
-
2.7.1.217
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Q8ZMG5
-
-
5.3.1.35
Cupriavidus necator
-
-
-
5.3.1.35
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Cupriavidus necator
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Cupriavidus necator
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Cupriavidus necator DSM 428
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.409
3-dehydro-4-phospho-L-threonate
-
741345
Cupriavidus necator
glycerone phosphate + CO2
spontaneous
-
-
?
1.1.1.409
3-dehydro-4-phospho-L-threonate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2
spontaneous
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
3-dehydro-4-phospho-L-threonate + NADH + H+
-
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-dehydro-4-phospho-L-threonate + NADH + H+
-
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.410
D-erythronate + NAD+
-
741345
Cupriavidus necator
2-dehydro-D-erythronate + NADH + H+
-
-
-
?
1.1.1.410
D-erythronate + NAD+
-
741345
Cupriavidus necator DSM 428
2-dehydro-D-erythronate + NADH + H+
-
-
-
?
1.1.1.410
D-erythronate + NADP+
-
741345
Cupriavidus necator
2-dehydro-D-erythronate + NADPH + H+
-
-
-
?
1.1.1.410
D-erythronate + NADP+
-
741345
Cupriavidus necator DSM 428
2-dehydro-D-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Cupriavidus necator
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Pectobacterium atrosepticum
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Pectobacterium atrosepticum ATCC BAA-672
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Cupriavidus necator
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Pectobacterium atrosepticum
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Pectobacterium atrosepticum ATCC BAA-672
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Cupriavidus necator DSM 428
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Cupriavidus necator
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Cupriavidus necator DSM 428
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Cupriavidus necator
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Cupriavidus necator DSM 428
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.408
0.35
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
0.37
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
3.4
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
8.9
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.410
3
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
19
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
2.2
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
4.7
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
29
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
54
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.410
NAD+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.410
NADP+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NAD+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NAD+
NAD+ is preferred over NADP+
Pectobacterium atrosepticum
1.1.1.411
NADP+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NADP+
NAD+ is preferred over NADP+
Pectobacterium atrosepticum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.410
NAD+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.410
NADP+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NAD+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NAD+
NAD+ is preferred over NADP+
Pectobacterium atrosepticum
1.1.1.411
NADP+
NAD+ is preferred over NADP+
Cupriavidus necator
1.1.1.411
NADP+
NAD+ is preferred over NADP+
Pectobacterium atrosepticum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.408
0.054
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
0.12
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
0.19
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
0.2
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
0.59
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
2.4
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.13
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
0.3
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.35
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
0.97
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Cupriavidus necator
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
3-dehydro-4-phospho-D-erythronate
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2
spontaneous reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Cupriavidus necator
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-hydroxy-L-threonine 4-phosphate + NAD+
-
741345
Cupriavidus necator DSM 428
3-amino-2-oxopropyl phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
3-dehydro-4-phospho-D-erythronate + NADH + H+
-
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.408
4-phospho-D-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.409
3-dehydro-4-phospho-L-threonate
-
741345
Cupriavidus necator
glycerone phosphate + CO2
spontaneous
-
-
?
1.1.1.409
3-dehydro-4-phospho-L-threonate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2
spontaneous
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
3-dehydro-4-phospho-L-threonate + NADH + H+
-
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
3-dehydro-4-phospho-L-threonate + NADH + H+
-
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Cupriavidus necator
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.409
4-phospho-D-erythronate + NAD+
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
glycerone phosphate + CO2 + NADH + H+
overall reaction
-
-
?
1.1.1.410
D-erythronate + NAD+
-
741345
Cupriavidus necator
2-dehydro-D-erythronate + NADH + H+
-
-
-
?
1.1.1.410
D-erythronate + NAD+
-
741345
Cupriavidus necator DSM 428
2-dehydro-D-erythronate + NADH + H+
-
-
-
?
1.1.1.410
D-erythronate + NADP+
-
741345
Cupriavidus necator
2-dehydro-D-erythronate + NADPH + H+
-
-
-
?
1.1.1.410
D-erythronate + NADP+
-
741345
Cupriavidus necator DSM 428
2-dehydro-D-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Cupriavidus necator
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Pectobacterium atrosepticum
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Pectobacterium atrosepticum ATCC BAA-672
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NAD+
-
741345
Cupriavidus necator DSM 428
2-dehydro-L-erythronate + NADH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Cupriavidus necator
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Pectobacterium atrosepticum
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Pectobacterium atrosepticum ATCC BAA-672
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
1.1.1.411
L-threonate + NADP+
-
741345
Cupriavidus necator DSM 428
2-dehydro-L-erythronate + NADPH + H+
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Cupriavidus necator
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-D-erythronate
-
741345
Cupriavidus necator DSM 428
ADP + 3-dehydro-4-phospho-D-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Cupriavidus necator
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
2.7.1.217
ATP + 3-dehydro-L-erythronate
-
741345
Cupriavidus necator DSM 428
ADP + 3-dehydro-4-phospho-L-erythronate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.408
0.35
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
0.37
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
3.4
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
8.9
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.410
3
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
19
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
2.2
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
4.7
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
29
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
54
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum
General Information
EC Number
General Information
Commentary
Organism
1.1.1.408
physiological function
members of the DUF1537 family are ATP-dependent kinases that participate in catabolic pathways for four-carbon acid sugars. PdxA2 participates in a pathway for the conversion of D-threonate to dihydroxyacetone phosphate and CO2. A strain lacking PdxA2 activity is unable to use D-threonate as a carbon source
Cupriavidus necator
2.7.1.217
physiological function
involved in utilization of L-threonate and D-erythronate, with different dehydrogenases generating the 2-oxo-tetronate that is isomerized to 3-oxo-tetronate by OtnI, phosphorylated to 3-oxo-tetronate 4-phosphate by OtnK, and decarboxylated to DHAP and CO2
Cupriavidus necator
2.7.1.217
physiological function
involved in utilization of L-threonate and D-erythronate, with different dehydrogenases generating the 2-oxo-tetronate that is isomerized to 3-oxo-tetronate by OtnI, phosphorylated to 3-oxo-tetronate 4-phosphate by OtnK, and decarboxylated to DHAP and CO2
Salmonella enterica subsp. enterica serovar Typhimurium
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.408
physiological function
members of the DUF1537 family are ATP-dependent kinases that participate in catabolic pathways for four-carbon acid sugars. PdxA2 participates in a pathway for the conversion of D-threonate to dihydroxyacetone phosphate and CO2. A strain lacking PdxA2 activity is unable to use D-threonate as a carbon source
Cupriavidus necator
2.7.1.217
physiological function
involved in utilization of L-threonate and D-erythronate, with different dehydrogenases generating the 2-oxo-tetronate that is isomerized to 3-oxo-tetronate by OtnI, phosphorylated to 3-oxo-tetronate 4-phosphate by OtnK, and decarboxylated to DHAP and CO2
Cupriavidus necator
2.7.1.217
physiological function
involved in utilization of L-threonate and D-erythronate, with different dehydrogenases generating the 2-oxo-tetronate that is isomerized to 3-oxo-tetronate by OtnI, phosphorylated to 3-oxo-tetronate 4-phosphate by OtnK, and decarboxylated to DHAP and CO2
Salmonella enterica subsp. enterica serovar Typhimurium
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.408
1.8
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
1.9
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
29
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
160
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.410
1.2
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
33
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
4.8
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
7.5
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
85
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
410
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.408
1.8
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
1.9
-
4-hydroxy-L-threonine 4-phosphate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.408
29
-
4-phospho-D-threonate
pH 8.0, 25°C
Cupriavidus necator
1.1.1.408
160
-
4-phospho-D-threonate
pH 8.0, 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.410
1.2
-
D-erythronate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.410
33
-
D-erythronate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
4.8
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Pectobacterium atrosepticum
1.1.1.411
7.5
-
L-threonate
cosubstrate NADP+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
85
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Cupriavidus necator
1.1.1.411
410
-
L-threonate
cosubstrate NAD+, pH 8.0, 25°C
Pectobacterium atrosepticum