Literature summary extracted from
Klebba, J.E.; Buster, D.W.; McLamarrah, T.A.; Rusan, N.M.; Rogers, G.C.
Autoinhibition and relief mechanism for polo-like kinase 4 (2015), Proc. Natl. Acad. Sci. USA, 112, E657-E666.
Cloned(Commentary)
| EC Number |
Cloned (Comment) |
Organism |
|---|
| 2.7.11.21 |
recombinant expression of full-length Plk4, subcloned into a pMT vector containing an in-frame coding sequence for EGFP or myc and the inducible metallothionein promoter, in Drosophila S2 cells, recombinant expression of the C-terminally FLAG-His6-tagged polo kinase domain of Plk4 in Escherichia coli strain BL21(DE3) |
Drosophila melanogaster |
Protein Variants
| EC Number |
Protein Variants |
Comment |
Organism |
|---|
| 2.7.11.21 |
additional information |
complete inactivation of a kinase domain mutant. Expression of Plk4 phospho-mutants influences centriole numbers |
Drosophila melanogaster |
| 2.7.11.21 |
S374A/S378A |
site-directed mutagenesis within L1 (GST-L1-Ala-602), the double mutant shows reduced enzyme activity compared to the wild-type enzyme |
Drosophila melanogaster |
Inhibitors
| EC Number |
Inhibitors |
Comment |
Organism |
Structure |
|---|
| 2.7.11.21 |
additional information |
Plk4 possesses an autoinhibitory mechanism mediated by a linker (L1) near the kinase domain, newly synthesized Plk4 is autoinhibited by L1. Autoinhibition is a conserved feature of Plks. In the case of Plk4, autoinhibition is relieved after homodimerization and is accomplished by PB3 and by autophosphorylation of L1. In contrast, autophosphorylation of the second linker promotes separation of the Plk4 homodimer. Mechanism for Plk4 autoinhibition, overview |
Drosophila melanogaster |
|
Metals/Ions
| EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
|---|
| 2.7.11.21 |
Mg2+ |
required |
Drosophila melanogaster |
|
| 2.7.11.21 |
Mn2+ |
required |
Drosophila melanogaster |
|
Organism
| EC Number |
Organism |
UniProt |
Comment |
Textmining |
|---|
| 2.7.11.21 |
Drosophila melanogaster |
O97143 |
- |
- |
Posttranslational Modification
| EC Number |
Posttranslational Modification |
Comment |
Organism |
|---|
| 2.7.11.21 |
phosphoprotein |
the enzyme performs autophosphorylation. Plk4 phosphorylation pattern, overview |
Drosophila melanogaster |
| 2.7.11.21 |
ubiquitination |
polo box 1, PB1, of Plk4 contains multiple ubiquitination sites. At least some of the Lys in PB1 are physiologically important targets of Plk4 ubiquitination, overview |
Drosophila melanogaster |
Purification (Commentary)
| EC Number |
Purification (Comment) |
Organism |
|---|
| 2.7.11.21 |
recombinant C-terminally FLAG-His6-tagged polo kinase domain of Plk4 fromherichia coli strain BL21(DE3) by nickel affinity chromatography |
Drosophila melanogaster |
Substrates and Products (Substrate)
| EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
|---|
| 2.7.11.21 |
additional information |
autophosphorylation of wild-type full-length enzyme and isolated kinase domain |
Drosophila melanogaster |
? |
- |
? |
|
Subunits
| EC Number |
Subunits |
Comment |
Organism |
|---|
| 2.7.11.21 |
homodimer |
the three polo boxes of Plk4 are crucial for Plk4 homodimerization |
Drosophila melanogaster |
Synonyms
| EC Number |
Synonyms |
Comment |
Organism |
|---|
| 2.7.11.21 |
for polo-like kinase 4 |
- |
Drosophila melanogaster |
| 2.7.11.21 |
PLK4 |
- |
Drosophila melanogaster |
Temperature Optimum [°C]
| EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
|---|
| 2.7.11.21 |
25 |
- |
assay at |
Drosophila melanogaster |
pH Optimum
| EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
|---|
| 2.7.11.21 |
7.3 |
- |
assay at |
Drosophila melanogaster |
Cofactor
| EC Number |
Cofactor |
Comment |
Organism |
Structure |
|---|
| 2.7.11.21 |
ATP |
- |
Drosophila melanogaster |
|
General Information
| EC Number |
General Information |
Comment |
Organism |
|---|
| 2.7.11.21 |
malfunction |
kinase activity is impaired in Plk4-DELTAPB3, polo box 3-mutated enzyme, reducing its ability to transautophosphorylate and recruit Slimb and thereby increasing its stability |
Drosophila melanogaster |
| 2.7.11.21 |
additional information |
the three polo boxes of Plk4 not only are crucial for Plk4 homodimerization and ubiquitination but also relieve autoinhibition caused by linker 1. Polo box 3 is required for kinase activity |
Drosophila melanogaster |
| 2.7.11.21 |
physiological function |
polo-like kinase 4 is a master regulator of centriole duplication, and its hyperactivity induces centriole amplification. Homodimeric Plk4 is ubiquitinated as a result of autophosphorylation, promoting its own degradation and preventing centriole amplification. Speculative multistep model for Plk4 activation and regulation, overview |
Drosophila melanogaster |
