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Literature summary extracted from

  • Sycz, G.; Carrica, M.C.; Tseng, T.S.; Bogomolni, R.A.; Briggs, W.R.; Goldbaum, F.A.; Paris, G.
    LOV histidine kinase modulates the general stress response system and affects the virB operon expression in Brucella abortus (2015), PLoS ONE, 10, e0124058.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.13.3 additional information exposure to light increases the autophosphorylation activity of the enzyme Brucella abortus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 gene BAB2_0652, recombinant expression f the enzyme in a two-hybrid system with PdhS in frame with the lambdacl gene, coexpression with gene pdhS, recombinant expression of His-tagged lovhk and hk domains in Escherichia coli strain BL21(DE3) pLysS from pET24a plasmid, coexpression of phyR and lovR. Antibiotic resistance complementation assays with the enzyme in Escherichia coli, overview. Realtime quantitative RT-PCR expression analysis Brucella abortus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 additional information construction of a LOVHK knockout mutant Brucella abortus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.13.3 Mg2+ required Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 ATP + protein L-histidine Brucella abortus
-
 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine Brucella abortus 2308
-
 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 additional information Brucella abortus LOVHK is able to phosphotransfer to the central regulator of the GSR system in Brucella, specific interaction with its cognate response regulators ?
-
 ?
2.7.13.3 additional information Brucella abortus 2308 LOVHK is able to phosphotransfer to the central regulator of the GSR system in Brucella, specific interaction with its cognate response regulators ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Brucella abortus Q2YKK7
-
-
2.7.13.3 Brucella abortus 2308 Q2YKK7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.13.3 phosphoprotein activation of LOVHK by blue-light increases the autophosphorylation activity of the HK domain of the histidine kinase Brucella abortus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 recombinant His-tagged LOVHK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 ATP + protein L-histidine
-
 Brucella abortus ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine
-
 Brucella abortus 2308 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 additional information LOVHK is able to phosphotransfer to the central regulator of the GSR system in Brucella, specific interaction with its cognate response regulators Brucella abortus ?
-
 ?
2.7.13.3 additional information LOVHK is able to phosphotransfer to the central regulator of the GSR system in Brucella, specific interaction with its cognate response regulators Brucella abortus 2308 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 BA-LOV-histidine kinase UniProt Brucella abortus
2.7.13.3 BA-LOV-HK UniProt Brucella abortus
2.7.13.3 BAB2_0652
-
 Brucella abortus
2.7.13.3 blue-light-activated histidine kinase UniProt Brucella abortus
2.7.13.3 LOV
-
 Brucella abortus
2.7.13.3 LOVHK
-
 Brucella abortus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 ATP
-
 Brucella abortus
2.7.13.3 additional information LOV domains are blue-light sensory modules which bind a flavin molecule as cofactor. Activation of LOVHK by blue-light increases the autophosphorylation activity of the HK domain Brucella abortus

General Information

EC Number General Information Comment Organism
2.7.13.3 evolution Brucella spp. genomes encode a LOV-domain-containing protein that is associated to a PAS (Per, Arnt and Sim) domain plus a HK domain (LOVHK), which belongs to the HWE family Brucella abortus
2.7.13.3 malfunction in the absence of LOVHK the expression of the virB operon is downregulated. LOVHK plays a role in the intracellular survival of Brucella, since deletion of this gene in Brucella abortus shows an attenuated phenotype in cell-culture infection assays. PhyR expression is decreased in the lovhk mutant Brucella abortus
2.7.13.3 additional information LOV domains are blue-light sensory modules which bind a flavin molecule as cofactor Brucella abortus
2.7.13.3 physiological function LOV histidine kinase positively regulates the general stress response system and affects the virB operon expression in Brucella abortus. The Brucella genome encodes a sensor histidine kinase containing a LOV domain upstream from the kinase, LOVHK, which plays an important role in light-regulated Brucella virulence. The intracellular signaling pathway is initiated by the light sensor LOVHK, which functionally interacts with two response regulators: PhyR and LovR, constituting a functional two-component signal-transduction system. LOVHK contributes to the activation of the general stress response (GSR) system in Brucella via PhyR, the main regulator of the general stress response system, while LovR is proposed to be a phosphate-sink for LOVHK, decreasing its phosphorylation state. LOVHK plays a role in the intracellular survival of Brucella and in defense of stress induced by starvation. LOVHK signaling cascade, overview Brucella abortus