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Literature summary extracted from

  • Li, H.; Ban, Z.; Qin, H.; Ma, L.; King, A.J.; Wang, G.
    A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway (2015), Plant Physiol., 167, 650-659.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.136 expression in Saccharomyces cerevisiae Humulus lupulus
2.5.1.137 expression in Saccharomyces cerevisiae Humulus lupulus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.136 additional information mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type Humulus lupulus
2.5.1.137 additional information mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type Humulus lupulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.136 0.00136
-
 phlorisovalerophenone truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.00339
-
 phlorisovalerophenone presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.0051
-
 phlorisobutyrophenone truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.0057
-
 geranyl diphosphate truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.00618
-
 geranyl diphosphate presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.00668
-
 phlorisobutyrophenone presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.0435
-
 dimethylallyl diphosphate truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
2.5.1.136 0.0436
-
 dimethylallyl diphosphate presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.137 chloroplast
-
 Humulus lupulus 9507
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.136 Humulus lupulus A0A0B5A051
-
-
2.5.1.137 Humulus lupulus A0A0B4ZTQ2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.136 dimethylallyl diphosphate + phlorisobutyrophenone
-
 Humulus lupulus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
2.5.1.136 dimethylallyl diphosphate + phlorisovalerophenone
-
 Humulus lupulus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
2.5.1.136 geranyl diphosphate + phlorisovalerophenone
-
 Humulus lupulus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-geranylphenyl]propan-1-one
-
 ?
2.5.1.137 dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Humulus lupulus diphosphate + ?
-
 ?
2.5.1.137 dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Humulus lupulus diphosphate + ?
-
 ?
2.5.1.137 dimethylallyl diphosphate + a 2-acyl-4,6-bisprenylphloroglucinol
-
 Humulus lupulus diphosphate + a 2-acyl-4,6,6-trisprenylcyclohexa-2,4-dien-1-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.136 aromatic prenyltransferase
-
 Humulus lupulus
2.5.1.136 PT1L
-
 Humulus lupulus
2.5.1.137 aromatic prenyltransferase
-
 Humulus lupulus
2.5.1.137 PT2
-
 Humulus lupulus

General Information

EC Number General Information Comment Organism
2.5.1.136 physiological function coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps Humulus lupulus
2.5.1.137 physiological function prenyltransferase PT2 catalyzes the last two prenylation steps in the beta-bitter acid pathway. Coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps Humulus lupulus