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Literature summary extracted from

  • Ito, T.; Fujimoto, S.; Suito, F.; Shimosaka, M.; Taguchi, G.
    C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants (2017), Plant J., 91, 187-198.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.360 expressed in Escherichia coli Rosetta 2(DE3) Citrus unshiu
2.4.1.360 expressed in Escherichia coli Rosetta 2(DE3) Citrus japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.360 0.00085
-
 2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 0.0144
-
 nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 0.0715
-
 UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
2.4.1.360 0.112
-
 6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.360 Citrus japonica
-
-
-
2.4.1.360 Citrus unshiu
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.360
-
 Citrus unshiu
2.4.1.360 purification via a nickel-affinity column, concentration using Amicon-Ultra-15 Ultracel-10k Citrus japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.360 flower
-
 Citrus japonica
-
2.4.1.360 leaf
-
 Citrus unshiu
-
2.4.1.360 leaf
-
 Citrus japonica
-
2.4.1.360 additional information detected in all plant organs Citrus unshiu
-
2.4.1.360 additional information detected in all plant organs Citrus japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.360 2 UDP-alpha-D-glucose + 2-hydroxynaringenin
-
 Citrus unshiu 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
 ?
2.4.1.360 2 UDP-alpha-D-glucose + 2-hydroxynaringenin
-
 Citrus japonica 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin
-
 ?
2.4.1.360 2 UDP-alpha-D-glucose + phloretin
-
 Citrus unshiu 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin
-
 ?
2.4.1.360 2 UDP-alpha-D-glucose + phloretin
-
 Citrus japonica 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin
-
 ?
2.4.1.360 additional information no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin Citrus unshiu ?
-
 ?
2.4.1.360 additional information no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin Citrus japonica ?
-
 ?
2.4.1.360 UDP-alpha-D-galactose + 2-hydroxypinocembrin low activity with sugar donor UDP-alpha-D-galactose Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-galactose + 2-hydroxypinocembrin low activity with sugar donor UDP-alpha-D-galactose Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone
-
 Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde
-
 Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone
-
 Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + maclurin
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + maclurin
-
 Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + nothofagin
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-glucose + nothofagin
-
 Citrus japonica UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin
-
 Citrus unshiu UDP + ?
-
 ?
2.4.1.360 UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin
-
 Citrus japonica UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.360 CuCGT
-
 Citrus unshiu
2.4.1.360 FcCGT
-
 Citrus japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.360 45
-
-
 Citrus unshiu
2.4.1.360 50
-
-
 Citrus japonica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.360 55
-
 inactivation above Citrus unshiu
2.4.1.360 60
-
 activity is significantly retained up to 60°C Citrus japonica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.360 0.8
-
 UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
2.4.1.360 5.3
-
 nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 5.7
-
 6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 7.6
-
 2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 12
-
 phloretin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.360 8 11 more than 65% of maximum activity in this range Citrus unshiu
2.4.1.360 8 11 more than 65% of maximum activity in this range Citrus japonica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.360 0.0089
-
 2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 0.037
-
 nothofagin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica
2.4.1.360 0.11
-
 UDP-alpha-D-glucose 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor Citrus japonica
2.4.1.360 0.51
-
 6-beta-D-glucopyranosyl-2-hydroxynaringenin 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C Citrus japonica