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Literature summary extracted from

  • Karamat, F.; Olry, A.; Munakata, R.; Koeduka, T.; Sugiyama, A.; Paris, C.; Hehn, A.; Bourgaud, F.; Yazaki, K.
    A coumarin-specific prenyltransferase catalyzes the crucial biosynthetic reaction for furanocoumarin formation in parsley (2014), Plant J., 77, 627-638.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.139 0.021
-
 umbelliferone product demethylsuberosin, pH 8.0, 25°C Petroselinum crispum
2.5.1.139 0.025
-
 umbelliferone product osthenol, pH 8.0, 25°C Petroselinum crispum
2.5.1.139 0.045
-
 dimethylallyl diphosphate product osthenol, pH 8.0, 25°C Petroselinum crispum
2.5.1.139 0.079
-
 dimethylallyl diphosphate product demethylsuberosin, pH 8.0, 25°C Petroselinum crispum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.139 plastid
-
 Petroselinum crispum 9536
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.139 Petroselinum crispum W0SKS3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.139 dimethylallyl diphosphate + umbelliferone
-
 Petroselinum crispum diphosphate + demethylsuberosin
-
 ?
2.5.1.139 dimethylallyl diphosphate + umbelliferone
-
 Petroselinum crispum diphosphate + osthenol minor product besides demethylsuberosin, about 5% of the amount of demethylsuberosin ?
2.5.1.139 additional information enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6 position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8-prenylated derivative (osthenol) is also formed, but to a much lesser extent Petroselinum crispum ?
-
 ?

Expression

EC Number Organism Comment Expression
2.5.1.139 Petroselinum crispum expression in various parsley tissues is increased by UV irradiation, with a concomitant increase in furanocoumarin production up

General Information

EC Number General Information Comment Organism
2.5.1.139 physiological function expression in the coumarin-producing plant Ruta graveolens shows increased consumption of endogenous umbelliferone. Expression of the prenyltransferase and a 4–coumaroyl CoA 2'–hydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, results in formation of demethylsuberosin Petroselinum crispum