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Literature summary extracted from
- Rapid purification and characterization of cystine lyase b from broccoli inflorescence (1999), Phytochemistry, 51, 853-859.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.35 | carboxymethoxylamine | 0.5 mM, 2% residual activity; 0.5 mM, complete inhibition | Brassica oleracea var. italica |  |
| 4.4.1.35 | hydroxylamine | 0.5 mM, 15% residual activity; 0.5 mM, complete inhibition | Brassica oleracea var. italica | |
| 4.4.1.35 | additional information | not inhibitory at 0.5 mM, iodoacetate, N-ethylmaleimide, EDTA; not inhibitory: iodoacetate, N-ethylmaleimide and EDTA | Brassica oleracea var. italica |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.4.1.35 | cytosol | and/or vacuole | Brassica oleracea var. italica | 5829 | - |
| 4.4.1.35 | vacuole | and/or cytosol | Brassica oleracea var. italica | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 40000 | - |
4 * 40000, SDS-PAGE | Brassica oleracea var. italica |
| 4.4.1.35 | 45000 | - |
4 * 45000, SDS-PAGE | Brassica oleracea var. italica |
| 4.4.1.35 | 160000 | - |
gel filtration | Brassica oleracea var. italica |
| 4.4.1.35 | 186000 | - |
gel filtration | Brassica oleracea var. italica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.35 | 2-aminoprop-2-enoate | Brassica oleracea var. italica | - |
2-iminopropanoate | spontaneous partial reaction | ? | |
| 4.4.1.35 | 2-iminopropanoate + H2O | Brassica oleracea var. italica | - |
pyruvate + NH3 | spontaneous partial reaction | ? | |
| 4.4.1.35 | L-cystine | Brassica oleracea var. italica | - |
L-thiocysteine + 2-aminoprop-2-enoate | - |
? | |
| 4.4.1.35 | L-cystine + H2O | Brassica oleracea var. italica | - |
L-thiocysteine + pyruvate + NH3 | overall reaction | ? | |
| 4.4.1.35 | additional information | Brassica oleracea var. italica | the enzyme catalyzes beta-elimination of L-cystine to yield thiocysteine, pyruvate and possibly ammonia. S-Methyl L-cysteine sulfoxide and S-ethyl L-cysteine sulfoxide are substrates but are less suitable than L-cystine. No substrates: D-cystine, L- and o-cysteine and cystathionine | ? | - |
? | |
| 4.4.1.35 | S-ethyl L-cysteine sulfoxide + H2O | Brassica oleracea var. italica | 48% of the activity with L-cystine | ? | - |
? | |
| 4.4.1.35 | S-methyl L-cysteine sulfoxide + H2O | Brassica oleracea var. italica | 37% of the activity with L-cystine | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.35 | Brassica oleracea var. italica | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.4.1.35 | no glycoprotein | - |
Brassica oleracea var. italica |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.35 | - |
Brassica oleracea var. italica |
| 4.4.1.35 | isoform cystine lyase b, rapid purifiaction method | Brassica oleracea var. italica |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.4.1.35 | inflorescence | - |
Brassica oleracea var. italica | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 29.8 | - |
pH 8.0, 30°C | Brassica oleracea var. italica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.35 | -20°C, little loss of activity during 6 months | Brassica oleracea var. italica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.35 | 2-aminoprop-2-enoate | - |
Brassica oleracea var. italica | 2-iminopropanoate | spontaneous partial reaction | ? | |
| 4.4.1.35 | 2-iminopropanoate + H2O | - |
Brassica oleracea var. italica | pyruvate + NH3 | spontaneous partial reaction | ? | |
| 4.4.1.35 | L-cystine | - |
Brassica oleracea var. italica | L-thiocysteine + 2-aminoprop-2-enoate | - |
? | |
| 4.4.1.35 | L-cystine + H2O | - |
Brassica oleracea var. italica | L-thiocysteine + pyruvate + NH3 | - |
? | |
| 4.4.1.35 | L-cystine + H2O | - |
Brassica oleracea var. italica | L-thiocysteine + pyruvate + NH3 | overall reaction | ? | |
| 4.4.1.35 | additional information | the enzyme catalyzes beta-elimination of L-cystine to yield thiocysteine, pyruvate and possibly ammonia. S-Methyl L-cysteine sulfoxide and S-ethyl L-cysteine sulfoxide are substrates but are less suitable than L-cystine. No substrates: D-cystine, L- and o-cysteine and cystathionine | Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-ethyl L-cysteine sulfoxide + H2O | - |
Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-ethyl L-cysteine sulfoxide + H2O | 48% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-ethyl L-cysteine sulfoxide + H2O | 64% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-methyl L-cysteine sulfoxide + H2O | - |
Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-methyl L-cysteine sulfoxide + H2O | 37% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
| 4.4.1.35 | S-methyl L-cysteine sulfoxide + H2O | 46% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.35 | tetramer | 4 * 45000, SDS-PAGE | Brassica oleracea var. italica |
| 4.4.1.35 | tetramer | 4 * 40000, SDS-PAGE | Brassica oleracea var. italica |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 30 | 40 | - |
Brassica oleracea var. italica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 40 | - |
5 min, stable | Brassica oleracea var. italica |
| 4.4.1.35 | 40 | - |
10 min, no loss of activity | Brassica oleracea var. italica |
| 4.4.1.35 | 50 | - |
5 min, 70% loss of activity | Brassica oleracea var. italica |
| 4.4.1.35 | 50 | - |
10 min, 60% loss of activity | Brassica oleracea var. italica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 8.5 | - |
- |
Brassica oleracea var. italica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.35 | 3 | 11 | more than 50% of maximum activity | Brassica oleracea var. italica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.35 | pyridoxal 5'-phosphate | - |
Brassica oleracea var. italica | |
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