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Literature summary extracted from

  • Zha, W.; Rubin-Pitel, S.B.; Zhao, H.
    Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity (2008), Mol. Biosyst., 4, 246-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.253 expressed in Escherichia coli Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.253 A289R the mutant shows 1.4fold improved catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
2.3.1.253 K210L the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
2.3.1.253 K210L/Y256R the mutant shows 0.9fold improved catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
2.3.1.253 Y256R the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens
2.3.1.253 Y256R/A289R the mutant shows 0.8fold improved catalytic efficiency compared to the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.253 0.0101
-
 malonyl-CoA mutant enzyme A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.0103
-
 malonyl-CoA mutant enzyme Y256R/A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.0112
-
 malonyl-CoA mutant enzyme Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.0119
-
 malonyl-CoA mutant enzyme K210L, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.0131
-
 malonyl-CoA wild type enzyme, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.0135
-
 malonyl-CoA mutant enzyme K210L/Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.253 77000
-
-
 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.253 3 malonyl-CoA Pseudomonas fluorescens
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
2.3.1.253 3 malonyl-CoA Pseudomonas fluorescens Pf-5
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.253 Pseudomonas fluorescens Q4K418
-
-
2.3.1.253 Pseudomonas fluorescens Pf-5 Q4K418
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.253 3 malonyl-CoA
-
 Pseudomonas fluorescens phloroglucinol + 3 CO2 + 3 CoA
-
 ?
2.3.1.253 3 malonyl-CoA
-
 Pseudomonas fluorescens Pf-5 phloroglucinol + 3 CO2 + 3 CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.253 homodimer
-
 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.253 PhlD
-
 Pseudomonas fluorescens
2.3.1.253 phloroglucinol synthase
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.253 37
-
 the enzyme has a half-life of 7.2 min at 37°C Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.253 0.08
-
 malonyl-CoA mutant enzyme Y256R/A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.11
-
 malonyl-CoA mutant enzyme K210L/Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.12
-
 malonyl-CoA wild type enzyme, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.13
-
 malonyl-CoA mutant enzyme A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.2
-
 malonyl-CoA mutant enzyme Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 0.21
-
 malonyl-CoA mutant enzyme K210L, pH and temperature not specified in the publication Pseudomonas fluorescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.253 7.5
-
 malonyl-CoA mutant enzyme Y256R/A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 8.3
-
 malonyl-CoA mutant enzyme K210L/Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 9.3
-
 malonyl-CoA wild type enzyme, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 12.8
-
 malonyl-CoA mutant enzyme A289R, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 17.5
-
 malonyl-CoA mutant enzyme K210L, pH and temperature not specified in the publication Pseudomonas fluorescens
2.3.1.253 17.7
-
 malonyl-CoA mutant enzyme Y256R, pH and temperature not specified in the publication Pseudomonas fluorescens