EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (Pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, pyrophosphate (all 1 mM) or ?H2PO4 (10 mM)) | Methylosinus trichosporium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.37 | gene mdh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Methylosinus trichosporium |
1.1.1.37 | gene mdh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Methylotuvimicrobium alcaliphilum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) | Methylosinus trichosporium | |
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (Pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, pyrophosphate (all 1 mM) or ?H2PO4 (10 mM)) | Methylotuvimicrobium alcaliphilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | additional information | - |
additional information | Michaelis-Menten kinetics | Methylosinus trichosporium | |
1.1.1.37 | additional information | - |
additional information | Michaelis-Menten kinetics | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 0.0252 | - |
NADH | recombinant enzyme, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 0.0376 | - |
NADH | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium | |
1.1.1.37 | 0.059 | - |
oxaloacetate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium | |
1.1.1.37 | 0.11 | - |
(S)-malate | recombinant enzyme, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 0.33 | - |
NAD+ | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium | |
1.1.1.37 | 0.34 | - |
oxaloacetate | recombinant enzyme, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 0.45 | - |
NAD+ | recombinant enzyme, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 1.28 | - |
(S)-malate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) | Methylosinus trichosporium | |
1.1.1.37 | additional information | the thermostable enzyme is not affected by metal ions (CuCl2, MgCl2, MnCl2, CoCl2, BaCl2, ZnCl2 or CaCl2 and NaCl or KCl) or various organic metabolites (pyruvate, phosphoenolpyruvate, ATP, ADP, AMP, glucose-1-phospate, fructose-1-phosphate, fructose-1,6-bisphosphate, fructose-6-phosphate, ribose-1-phosphate, ribose-5-phosphate (all 5 mM), glycerate, lactate, 2-oxoglutarate, citrate, serine, diphosphate (all 1 mM) or phosphate (10 mM)) | Methylotuvimicrobium alcaliphilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 33200 | - |
- |
Methylosinus trichosporium |
1.1.1.37 | 35000 | - |
- |
Methylosinus trichosporium |
1.1.1.37 | 35000 | - |
- |
Methylotuvimicrobium alcaliphilum |
1.1.1.37 | 35800 | - |
- |
Methylotuvimicrobium alcaliphilum |
1.1.1.37 | 70000 | - |
recombinant enzyme, gel filtration | Methylotuvimicrobium alcaliphilum |
1.1.1.37 | 140000 | - |
recombinant enzyme, gel filtration | Methylosinus trichosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + NAD+ | Methylosinus trichosporium | - |
oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | Methylotuvimicrobium alcaliphilum | - |
oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | Methylotuvimicrobium alcaliphilum 20Z | - |
oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | Methylosinus trichosporium | - |
(S)-malate + NAD+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | Methylotuvimicrobium alcaliphilum | - |
(S)-malate + NAD+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | Methylotuvimicrobium alcaliphilum 20Z | - |
(S)-malate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.37 | Methylosinus trichosporium | - |
- |
- |
1.1.1.37 | Methylotuvimicrobium alcaliphilum | G4T3S9 | - |
- |
1.1.1.37 | Methylotuvimicrobium alcaliphilum 20Z | G4T3S9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.37 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Methylosinus trichosporium |
1.1.1.37 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 15.05 | - |
purified recombinant enzyme, oxaloacetate reduction, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum |
1.1.1.37 | 20.75 | - |
purified recombinant enzyme, malate oxidation, pH 10.0, 30°C | Methylotuvimicrobium alcaliphilum |
1.1.1.37 | 78.2 | - |
purified recombinant enzyme, malate oxidation, pH 9.5, 30°C | Methylosinus trichosporium |
1.1.1.37 | 187.8 | - |
purified recombinant enzyme, oxaloacetate reduction, pH 9.5, 30°C | Methylosinus trichosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + NAD+ | - |
Methylosinus trichosporium | oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | - |
Methylotuvimicrobium alcaliphilum | oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | (S)-malate + NAD+ | - |
Methylotuvimicrobium alcaliphilum 20Z | oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | additional information | the enzyme displays nearly equal reductive (malate formation) and oxidative (oxaloacetate formation) activities and higher affinity to malate than to oxaloacetate | Methylotuvimicrobium alcaliphilum | ? | - |
? | |
1.1.1.37 | additional information | the enzyme is 2fold more active in the reaction of oxaloacetate reduction compared to malate oxidation and exhibiting higher affinity to oxaloacetate than to malate | Methylosinus trichosporium | ? | - |
? | |
1.1.1.37 | additional information | the enzyme displays nearly equal reductive (malate formation) and oxidative (oxaloacetate formation) activities and higher affinity to malate than to oxaloacetate | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
? | |
1.1.1.37 | oxaloacetate + NADH + H+ | - |
Methylosinus trichosporium | (S)-malate + NAD+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | - |
Methylotuvimicrobium alcaliphilum | (S)-malate + NAD+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | - |
Methylotuvimicrobium alcaliphilum 20Z | (S)-malate + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.37 | homodimer | 2 * 35000, recombinant enzyme, SDS-PAGE, 2 * 35800, about, sequence calculation | Methylotuvimicrobium alcaliphilum |
1.1.1.37 | homotetramer | 4 * 35000, recombinant enzyme, SDS-PAGE, 4 * 33200, about, sequence calculation | Methylosinus trichosporium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.37 | A8B73_11505 | - |
Methylosinus trichosporium |
1.1.1.37 | L-malate: NAD oxidoreductase | - |
Methylosinus trichosporium |
1.1.1.37 | L-malate: NAD oxidoreductase | - |
Methylotuvimicrobium alcaliphilum |
1.1.1.37 | MDH | - |
Methylosinus trichosporium |
1.1.1.37 | MDH | - |
Methylotuvimicrobium alcaliphilum |
1.1.1.37 | NAD+-dependent malate dehydrogenase | - |
Methylosinus trichosporium |
1.1.1.37 | NAD+-dependent malate dehydrogenase | - |
Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 60 | - |
- |
Methylosinus trichosporium |
1.1.1.37 | 60 | 65 | - |
Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 30 | 60 | the activity of the methanotroph's enzyme increases up to 60°? and then gradually decreases | Methylosinus trichosporium |
1.1.1.37 | 30 | 60 | the activity of the methanotroph's enzyme increases up to 60°? and then gradually decreases | Methylotuvimicrobium alcaliphilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 30 | 70 | the enzyme is active in a broader temperature range. It retains full activity after 18 h exposure at 30-60°C, exhibiting a 1 h half-life time at 70°C | Methylosinus trichosporium |
1.1.1.37 | 30 | 60 | the purified recombinant His6-tagged MDH from strain 20Z fully retains activity after 18 h exposure at 30-40°C, and has a 10% residual activity after 18 h exposure at 50°C. The enzyme loses 50% of its activity after 10 min heating at 60°C | Methylotuvimicrobium alcaliphilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 516 | - |
oxaloacetate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium | |
1.1.1.37 | 621 | - |
(S)-malate | recombinant enzyme, pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 870 | - |
oxaloacetate | recombinant enzyme, pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 1141 | - |
(S)-malate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 9.5 | - |
- |
Methylosinus trichosporium |
1.1.1.37 | 10 | - |
- |
Methylotuvimicrobium alcaliphilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.37 | 7.5 | 10 | activity range, recombinant enzyme | Methylosinus trichosporium |
1.1.1.37 | 7.5 | 10 | activity range, recombinant enzyme | Methylotuvimicrobium alcaliphilum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.37 | NAD+ | - |
Methylosinus trichosporium | |
1.1.1.37 | NAD+ | - |
Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | NADH | - |
Methylosinus trichosporium | |
1.1.1.37 | NADH | - |
Methylotuvimicrobium alcaliphilum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.37 | metabolism | the enzyme plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis | Methylosinus trichosporium |
1.1.1.37 | metabolism | the enzyme plays crucial roles in many metabolic pathways, including the tricarboxylic acid (TCA) cycle, energy generation and the formation of metabolites for biosynthesis | Methylotuvimicrobium alcaliphilum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 403 | - |
(S)-malate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium | |
1.1.1.37 | 2558 | - |
oxaloacetate | recombinant enzyme, pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 5648 | - |
(S)-malate | recombinant enzyme, pH 9.5, 30°C | Methylotuvimicrobium alcaliphilum | |
1.1.1.37 | 19340 | - |
oxaloacetate | recombinant enzyme, pH 9.5, 30°C | Methylosinus trichosporium |