Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, C.M.; Chen, S.M.; Chien, P.J.; Yu, H.Y.
    Development of an enzymatic assay system of D-lactate using D-lactate dehydrogenase and a UV-LED fluorescent spectrometer (2015), J. Pharm. Biomed. Anal., 116, 150-155.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Rattus norvegicus
-
 pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ Rattus norvegicus Wistar
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Rattus norvegicus A0A0G2K1W9
-
-
1.1.1.28 Rattus norvegicus Wistar A0A0G2K1W9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.28 blood serum
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Rattus norvegicus pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ development of an assay system of D-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) is utilized to catalyze D-lactate and NAD+ to pyruvate and NADH, analysis of D-lactate by HPLC, respectively. Structure of the enzymatic assay system with a UV-LED fluorescent spectrometer, and method optimization and validation, overview Rattus norvegicus pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Rattus norvegicus Wistar pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ development of an assay system of D-lactate with good precision, accuracy, and sensitivity for the determination of D-lactate concentrations in rat serum. D-Lactate dehydrogenase (D-LDH) is utilized to catalyze D-lactate and NAD+ to pyruvate and NADH, analysis of D-lactate by HPLC, respectively. Structure of the enzymatic assay system with a UV-LED fluorescent spectrometer, and method optimization and validation, overview Rattus norvegicus Wistar pyruvate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-LDH
-
 Rattus norvegicus
1.1.1.28 ldhd
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 25
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 8.5
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 cytochrome c
-
 Rattus norvegicus
1.1.1.28 FAD
-
 Rattus norvegicus
1.1.1.28 NAD+
-
 Rattus norvegicus