BRENDA - Enzyme Database

Novel alcohol oxidase with glycolate oxidase activity from Ochrobactrum sp. AIU 033

Yamada, M.; Higashiyama, T.; Kishino, S.; Kataoka, M.; Ogawa, J.; Shimizu, S.; Isobe, K.; J. Mol. Catal. B 105, 41-48 (2014)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
EDTA
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydrazine
89% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydroxylamine
72% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
iodoacetic acid
59% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
KCN
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Mg2+
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
N-ethylmaleimide
84% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
o-phenanthroline
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
phenylhydrazine
1% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Semicarbazide
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Sodium azide
97% residual activity at 1 mM
Ochrobactrum sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.0109
-
1-Decanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
0.0365
-
1-Octanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
0.41
-
1-Hexanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
5.54
-
1-Pentanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
15.8
-
Lactate
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
167
-
glycolate
at pH 5.5 and 30C
Ochrobactrum sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
Fe3+
the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+
Ochrobactrum sp.
1.1.3.13
additional information
the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+
Ochrobactrum sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
14000
-
-
Ochrobactrum sp.
1.1.3.13
52000
-
-
Ochrobactrum sp.
1.1.3.13
130000
-
gel filtration
Ochrobactrum sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.3.13
Ochrobactrum sp.
-
-
-
1.1.3.13
Ochrobactrum sp. AIU 033
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.3.13
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography
Ochrobactrum sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.3.13
0.0407
-
cell-free extract, with glycolate as substrate, at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
4.02
-
after 101fold purification, with glycolate as substrate, at pH 5.5 and 30C
Ochrobactrum sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp.
butanal + H2O2
-
-
-
?
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
butanal + H2O2
-
-
-
?
1.1.3.13
1-decanol + O2
180% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
1-hexanol + O2
300% activity compared to glycolate
740872
Ochrobactrum sp.
hexanal + H2O2
-
-
-
?
1.1.3.13
1-octanol + O2
189% activity compared to glycolate
740872
Ochrobactrum sp.
octanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
448% activity compared to glycolate
740872
Ochrobactrum sp.
pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp.
propanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
propanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp.
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp.
glyoxylate + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp. AIU 033
glyoxylate + H2O2
-
-
-
?
1.1.3.13
lactate + O2
14% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp.
?
-
-
-
-
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp. AIU 033
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
heterotetramer
2 * 52000 + 2 * 14000, SDS-PAGE
Ochrobactrum sp.
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.3.13
AOX
-
Ochrobactrum sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.3.13
45
-
-
Ochrobactrum sp.
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.3.13
25
45
the enzyme exhibits more than 60% activity between 25 and 45C, and no activity at 50C
Ochrobactrum sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
5.5
-
-
Ochrobactrum sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
5.5
6.5
more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5
Ochrobactrum sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Ochrobactrum sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Ochrobactrum sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
EDTA
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydrazine
89% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydroxylamine
72% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
iodoacetic acid
59% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
KCN
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Mg2+
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
N-ethylmaleimide
84% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
o-phenanthroline
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
phenylhydrazine
1% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Semicarbazide
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Sodium azide
97% residual activity at 1 mM
Ochrobactrum sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.0109
-
1-Decanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
0.0365
-
1-Octanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
0.41
-
1-Hexanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
5.54
-
1-Pentanol
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
15.8
-
Lactate
at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
167
-
glycolate
at pH 5.5 and 30C
Ochrobactrum sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
Fe3+
the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+
Ochrobactrum sp.
1.1.3.13
additional information
the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+
Ochrobactrum sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
14000
-
-
Ochrobactrum sp.
1.1.3.13
52000
-
-
Ochrobactrum sp.
1.1.3.13
130000
-
gel filtration
Ochrobactrum sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography
Ochrobactrum sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.3.13
0.0407
-
cell-free extract, with glycolate as substrate, at pH 5.5 and 30C
Ochrobactrum sp.
1.1.3.13
4.02
-
after 101fold purification, with glycolate as substrate, at pH 5.5 and 30C
Ochrobactrum sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp.
butanal + H2O2
-
-
-
?
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
butanal + H2O2
-
-
-
?
1.1.3.13
1-decanol + O2
180% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
1-hexanol + O2
300% activity compared to glycolate
740872
Ochrobactrum sp.
hexanal + H2O2
-
-
-
?
1.1.3.13
1-octanol + O2
189% activity compared to glycolate
740872
Ochrobactrum sp.
octanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
448% activity compared to glycolate
740872
Ochrobactrum sp.
pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp.
propanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
propanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp.
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp.
glyoxylate + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp. AIU 033
glyoxylate + H2O2
-
-
-
?
1.1.3.13
lactate + O2
14% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp.
?
-
-
-
-
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp. AIU 033
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
heterotetramer
2 * 52000 + 2 * 14000, SDS-PAGE
Ochrobactrum sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.3.13
45
-
-
Ochrobactrum sp.
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.3.13
25
45
the enzyme exhibits more than 60% activity between 25 and 45C, and no activity at 50C
Ochrobactrum sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
5.5
-
-
Ochrobactrum sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
5.5
6.5
more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5
Ochrobactrum sp.