BRENDA - Enzyme Database show

Novel alcohol oxidase with glycolate oxidase activity from Ochrobactrum sp. AIU 033

Yamada, M.; Higashiyama, T.; Kishino, S.; Kataoka, M.; Ogawa, J.; Shimizu, S.; Isobe, K.; J. Mol. Catal. B 105, 41-48 (2014)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
EDTA
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydrazine
89% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydroxylamine
72% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
iodoacetic acid
59% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
KCN
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Mg2+
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
N-ethylmaleimide
84% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
o-phenanthroline
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
phenylhydrazine
1% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Semicarbazide
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Sodium azide
97% residual activity at 1 mM
Ochrobactrum sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.0109
-
1-Decanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
0.0365
-
1-Octanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
0.41
-
1-Hexanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
5.54
-
1-Pentanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
15.8
-
Lactate
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
167
-
glycolate
at pH 5.5 and 30°C
Ochrobactrum sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
Fe3+
the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+
Ochrobactrum sp.
1.1.3.13
additional information
the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+
Ochrobactrum sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
14000
-
-
Ochrobactrum sp.
1.1.3.13
52000
-
-
Ochrobactrum sp.
1.1.3.13
130000
-
gel filtration
Ochrobactrum sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.13
Ochrobactrum sp.
-
-
-
1.1.3.13
Ochrobactrum sp. AIU 033
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.13
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography
Ochrobactrum sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
0.0407
-
cell-free extract, with glycolate as substrate, at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
4.02
-
after 101fold purification, with glycolate as substrate, at pH 5.5 and 30°C
Ochrobactrum sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp.
butanal + H2O2
-
-
-
?
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
butanal + H2O2
-
-
-
?
1.1.3.13
1-decanol + O2
180% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
1-hexanol + O2
300% activity compared to glycolate
740872
Ochrobactrum sp.
hexanal + H2O2
-
-
-
?
1.1.3.13
1-octanol + O2
189% activity compared to glycolate
740872
Ochrobactrum sp.
octanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
448% activity compared to glycolate
740872
Ochrobactrum sp.
pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp.
propanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
propanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp.
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp.
glyoxylate + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp. AIU 033
glyoxylate + H2O2
-
-
-
?
1.1.3.13
lactate + O2
14% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp.
?
-
-
-
-
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp. AIU 033
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
heterotetramer
2 * 52000 + 2 * 14000, SDS-PAGE
Ochrobactrum sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
45
-
-
Ochrobactrum sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.13
25
45
the enzyme exhibits more than 60% activity between 25 and 45°C, and no activity at 50°C
Ochrobactrum sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
5.5
-
-
Ochrobactrum sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
5.5
6.5
more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5
Ochrobactrum sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Ochrobactrum sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Ochrobactrum sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.13
Cu2+
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
EDTA
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydrazine
89% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
hydroxylamine
72% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
iodoacetic acid
59% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
KCN
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Mg2+
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
N-ethylmaleimide
84% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
o-phenanthroline
93% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
phenylhydrazine
1% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Semicarbazide
91% residual activity at 1 mM
Ochrobactrum sp.
1.1.3.13
Sodium azide
97% residual activity at 1 mM
Ochrobactrum sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
0.0109
-
1-Decanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
0.0365
-
1-Octanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
0.41
-
1-Hexanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
5.54
-
1-Pentanol
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
15.8
-
Lactate
at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
167
-
glycolate
at pH 5.5 and 30°C
Ochrobactrum sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.13
Fe3+
the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+
Ochrobactrum sp.
1.1.3.13
additional information
the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+
Ochrobactrum sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
14000
-
-
Ochrobactrum sp.
1.1.3.13
52000
-
-
Ochrobactrum sp.
1.1.3.13
130000
-
gel filtration
Ochrobactrum sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography
Ochrobactrum sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
0.0407
-
cell-free extract, with glycolate as substrate, at pH 5.5 and 30°C
Ochrobactrum sp.
1.1.3.13
4.02
-
after 101fold purification, with glycolate as substrate, at pH 5.5 and 30°C
Ochrobactrum sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp.
butanal + H2O2
-
-
-
?
1.1.3.13
1-butanol + O2
373% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
butanal + H2O2
-
-
-
?
1.1.3.13
1-decanol + O2
180% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
1-hexanol + O2
300% activity compared to glycolate
740872
Ochrobactrum sp.
hexanal + H2O2
-
-
-
?
1.1.3.13
1-octanol + O2
189% activity compared to glycolate
740872
Ochrobactrum sp.
octanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
448% activity compared to glycolate
740872
Ochrobactrum sp.
pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp.
propanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
63% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
propanal + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp.
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
10% activity compared to glycolate
740872
Ochrobactrum sp. AIU 033
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp.
glyoxylate + H2O2
-
-
-
?
1.1.3.13
glycolate + O2
100% activity
740872
Ochrobactrum sp. AIU 033
glyoxylate + H2O2
-
-
-
?
1.1.3.13
lactate + O2
14% activity compared to glycolate
740872
Ochrobactrum sp.
decanal + H2O2
-
-
-
?
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp.
?
-
-
-
-
1.1.3.13
additional information
the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol
740872
Ochrobactrum sp. AIU 033
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
heterotetramer
2 * 52000 + 2 * 14000, SDS-PAGE
Ochrobactrum sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.13
45
-
-
Ochrobactrum sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.13
25
45
the enzyme exhibits more than 60% activity between 25 and 45°C, and no activity at 50°C
Ochrobactrum sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
5.5
-
-
Ochrobactrum sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
5.5
6.5
more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5
Ochrobactrum sp.