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Literature summary extracted from

  • Yamada, M.; Higashiyama, T.; Kishino, S.; Kataoka, M.; Ogawa, J.; Shimizu, S.; Isobe, K.
    Novel alcohol oxidase with glycolate oxidase activity from Ochrobactrum sp. AIU 033 (2014), J. Mol. Catal. B, 105, 41-48.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 Cu2+ 91% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 EDTA 91% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 hydrazine 89% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 hydroxylamine 72% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 iodoacetic acid 59% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 KCN 93% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 Mg2+ 93% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 N-ethylmaleimide 84% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 o-phenanthroline 93% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 phenylhydrazine 1% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 Semicarbazide 91% residual activity at 1 mM Ochrobactrum sp.
1.1.3.13 Sodium azide 97% residual activity at 1 mM Ochrobactrum sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.0109
-
 1-Decanol at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 0.0365
-
 1-Octanol at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 0.41
-
 1-Hexanol at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 5.54
-
 1-Pentanol at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 15.8
-
 Lactate at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 167
-
 glycolate at pH 5.5 and 30°C Ochrobactrum sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.13 Fe3+ the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+ Ochrobactrum sp.
1.1.3.13 additional information the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+ Ochrobactrum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 14000
-
-
 Ochrobactrum sp.
1.1.3.13 52000
-
-
 Ochrobactrum sp.
1.1.3.13 130000
-
 gel filtration Ochrobactrum sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Ochrobactrum sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13 ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography Ochrobactrum sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 0.0407
-
 cell-free extract, with glycolate as substrate, at pH 5.5 and 30°C Ochrobactrum sp.
1.1.3.13 4.02
-
 after 101fold purification, with glycolate as substrate, at pH 5.5 and 30°C Ochrobactrum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1-butanol + O2 373% activity compared to glycolate Ochrobactrum sp. butanal + H2O2
-
 ?
1.1.3.13 1-decanol + O2 180% activity compared to glycolate Ochrobactrum sp. decanal + H2O2
-
 ?
1.1.3.13 1-hexanol + O2 300% activity compared to glycolate Ochrobactrum sp. hexanal + H2O2
-
 ?
1.1.3.13 1-octanol + O2 189% activity compared to glycolate Ochrobactrum sp. octanal + H2O2
-
 ?
1.1.3.13 1-pentanol + O2 448% activity compared to glycolate Ochrobactrum sp. pentanal + H2O2
-
 ?
1.1.3.13 1-propanol + O2 63% activity compared to glycolate Ochrobactrum sp. propanal + H2O2
-
 ?
1.1.3.13 ethanol + O2 10% activity compared to glycolate Ochrobactrum sp. acetaldehyde + H2O2
-
 ?
1.1.3.13 glycolate + O2 100% activity Ochrobactrum sp. glyoxylate + H2O2
-
 ?
1.1.3.13 lactate + O2 14% activity compared to glycolate Ochrobactrum sp. decanal + H2O2
-
 ?
1.1.3.13 additional information the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol Ochrobactrum sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 heterotetramer 2 * 52000 + 2 * 14000, SDS-PAGE Ochrobactrum sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOX
-
 Ochrobactrum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 45
-
-
 Ochrobactrum sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.13 25 45 the enzyme exhibits more than 60% activity between 25 and 45°C, and no activity at 50°C Ochrobactrum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 5.5
-
-
 Ochrobactrum sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.13 5.5 6.5 more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5 Ochrobactrum sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD
-
 Ochrobactrum sp.