BRENDA - Enzyme Database

Identification and characterization of D-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillus

Komeda, H.; Yamasaki-Yashiki, S.; Hoshino, K.; Asano, Y.; J. Biosci. Bioeng. 119, 57-64 (2015)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.307
0.0094
-
NADPH
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
0.35
-
NADH
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
28.1
-
D-xylose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
36.3
-
D-ribose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
41.7
-
L-arabinose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
72.2
-
D-galactose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
177
-
D-glucose
pH 7.0, 30°C
Rhizomucor pusillus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.307
38000
-
-
Rhizomucor pusillus
1.1.1.307
59000
-
gel filtration
Rhizomucor pusillus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.307
Rhizomucor pusillus
I7HD84
-
-
1.1.1.307
Rhizomucor pusillus NBRC 4578
I7HD84
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.307
from mycelium
Rhizomucor pusillus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.307
mycelium
-
Rhizomucor pusillus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.307
D-galactose + NADH + H+
53% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NAD+
-
-
-
?
1.1.1.307
D-galactose + NADH + H+
53% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NAD+
-
-
-
?
1.1.1.307
D-glucose + NADPH + H+
38% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NADP+
-
-
-
?
1.1.1.307
D-glucose + NADPH + H+
38% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NADP+
-
-
-
?
1.1.1.307
D-ribose + NADPH + H+
84% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NADP+
-
-
-
?
1.1.1.307
D-ribose + NADPH + H+
84% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NADP+
-
-
-
?
1.1.1.307
D-xylose + NADPH + H+
-
740777
Rhizomucor pusillus
xylitol + NADP+
-
-
-
?
1.1.1.307
D-xylose + NADPH + H+
-
740777
Rhizomucor pusillus NBRC 4578
xylitol + NADP+
-
-
-
?
1.1.1.307
L-arabinose + NADPH + H+
79% of the activity with D-xylose
740777
Rhizomucor pusillus
L-arabinitol + NADP+
-
-
-
?
1.1.1.307
L-arabinose + NADPH + H+
79% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
L-arabinitol + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.307
dimer
2 * 36724, calculated, 2 * 38000, SDS-PAGE
Rhizomucor pusillus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.307
21.3
-
D-xylose
pH 7.0, 30°C
Rhizomucor pusillus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.307
5
-
-
Rhizomucor pusillus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.307
NADH
2.4% of the activity with NADPH
Rhizomucor pusillus
1.1.1.307
NADPH
-
Rhizomucor pusillus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.307
NADH
2.4% of the activity with NADPH
Rhizomucor pusillus
1.1.1.307
NADPH
-
Rhizomucor pusillus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.307
0.0094
-
NADPH
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
0.35
-
NADH
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
28.1
-
D-xylose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
36.3
-
D-ribose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
41.7
-
L-arabinose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
72.2
-
D-galactose
pH 7.0, 30°C
Rhizomucor pusillus
1.1.1.307
177
-
D-glucose
pH 7.0, 30°C
Rhizomucor pusillus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.307
38000
-
-
Rhizomucor pusillus
1.1.1.307
59000
-
gel filtration
Rhizomucor pusillus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.307
from mycelium
Rhizomucor pusillus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.307
mycelium
-
Rhizomucor pusillus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.307
D-galactose + NADH + H+
53% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NAD+
-
-
-
?
1.1.1.307
D-galactose + NADH + H+
53% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NAD+
-
-
-
?
1.1.1.307
D-glucose + NADPH + H+
38% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NADP+
-
-
-
?
1.1.1.307
D-glucose + NADPH + H+
38% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NADP+
-
-
-
?
1.1.1.307
D-ribose + NADPH + H+
84% of the activity with D-xylose
740777
Rhizomucor pusillus
? + NADP+
-
-
-
?
1.1.1.307
D-ribose + NADPH + H+
84% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
? + NADP+
-
-
-
?
1.1.1.307
D-xylose + NADPH + H+
-
740777
Rhizomucor pusillus
xylitol + NADP+
-
-
-
?
1.1.1.307
D-xylose + NADPH + H+
-
740777
Rhizomucor pusillus NBRC 4578
xylitol + NADP+
-
-
-
?
1.1.1.307
L-arabinose + NADPH + H+
79% of the activity with D-xylose
740777
Rhizomucor pusillus
L-arabinitol + NADP+
-
-
-
?
1.1.1.307
L-arabinose + NADPH + H+
79% of the activity with D-xylose
740777
Rhizomucor pusillus NBRC 4578
L-arabinitol + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.307
dimer
2 * 36724, calculated, 2 * 38000, SDS-PAGE
Rhizomucor pusillus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.307
21.3
-
D-xylose
pH 7.0, 30°C
Rhizomucor pusillus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.307
5
-
-
Rhizomucor pusillus
Expression
EC Number
Organism
Commentary
Expression
1.1.1.307
Rhizomucor pusillus
transcription of the xyl1 gene is marked in the presence of D-xylose and L-arabinose, but is week in the presence of D-glucose
additional information
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.1.1.307
Rhizomucor pusillus
transcription of the xyl1 gene is marked in the presence of D-xylose and L-arabinose, but is week in the presence of D-glucose
additional information