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Literature summary extracted from

  • Mahmud, A.; Hattori, K.; Hongwen, C.; Kitamoto, N.; Suzuki, T.; Nakamura, K.; Takamizawa, K.
    Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and L-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae (2013), J. Biosci. Bioeng., 115, 353-359.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xdhA Aspergillus oryzae
1.1.1.12 gene ladA Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 additional information construction of deletion mutants of xdhA by homologous transformation, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Gene pyrG is used as a selectable marker. Consumption of D-xylose for xdhA2-1 and ladA2-1 is similar to that of KBN616. Mutant xdhA2-1 displays the highest xylitol productivity. XdhA-disrupted mutant xdhA2-1 exhibits the highest xylitol productivity from D-xylose and is selected for further production of xylitol from oat spelt xylan Aspergillus oryzae
1.1.1.12 additional information construction of deletion mutants of ladA by homologous transformation, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Gene pyrG is used as a selectable marker. Consumption of D-xylose for xdhA2-1 and ladA2-1 is similar to that of KBN616. Mutant ladA2-1 displays the reduced xylitol productivity Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Aspergillus oryzae
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+ Aspergillus oryzae P5
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+ Aspergillus oryzae
-
 L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+ Aspergillus oryzae P5
-
 L-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Aspergillus oryzae Q86ZV0 a DELTApyrG strain developed from strain KBN616
-
1.1.1.9 Aspergillus oryzae P5 Q86ZV0 a DELTApyrG strain developed from strain KBN616
-
1.1.1.12 Aspergillus oryzae Q763T4 a DELTApyrG strain developed from strain KBN616
-
1.1.1.12 Aspergillus oryzae P5 Q763T4 a DELTApyrG strain developed from strain KBN616
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae P5 D-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus oryzae L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus oryzae P5 L-xylulose + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 D-xylulose reductase A
-
 Aspergillus oryzae
1.1.1.9 xdhA
-
 Aspergillus oryzae
1.1.1.9 xylitol dehydrogenase
-
 Aspergillus oryzae
1.1.1.12 L-arabitol dehydrogenase
-
 Aspergillus oryzae
1.1.1.12 LADA
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 25
-
 assay at Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 7.5
-
 assay at Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Aspergillus oryzae
1.1.1.12 NAD+
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
1.1.1.9 additional information xylitol production of wild-type and mutant strains, overview Aspergillus oryzae
1.1.1.12 additional information xylitol production of wild-type and mutant strains, overview Aspergillus oryzae