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Literature summary extracted from

  • Morris, J.S.; Facchini, P.J.
    Isolation and characterization of reticuline N-methyltransferase involved in biosynthesis of the aporphine alkaloid magnoflorine in opium poppy (2016), J. Biol. Chem., 291, 23416-23427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.337 expression in Escherichia coli Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.337 0.042
-
 (S)-reticuline pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.085
-
 (R)-reticuline pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.168
-
 S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.337 41900
-
-
 Papaver somniferum
2.1.1.337 45000
-
-
 Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.337 additional information Papaver somniferum RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (+)-bulbocapnine Papaver somniferum 75% substrate conversion S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (+)-glaucine Papaver somniferum 47% substrate conversion S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (R)-reticuline Papaver somniferum 98% substrate conversion S-adenosyl-L-homocysteine + (S)-tembetarine
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (S)-corytuberine Papaver somniferum 82% substrate conversion S-adenosyl-L-homocysteine + magnoflorine
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (S)-reticuline Papaver somniferum 47% substrate conversion S-adenosyl-L-homocysteine + (S)-tembetarine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.337 Papaver somniferum A0A1C9U5X5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.337 root highest transcript level Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.337 additional information RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations Papaver somniferum ?
-
 ?
2.1.1.337 additional information RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations Papaver somniferum ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (+)-bulbocapnine 75% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (+)-glaucine 47% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (R)-reticuline 98% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + (S)-tembetarine
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (R,S)-tetrahydropapaverine 24% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (S)-corytuberine 82% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + magnoflorine
-
 ?
2.1.1.337 S-adenosyl-L-methionine + (S)-reticuline 47% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + (S)-tembetarine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.337 ? x * 41900, calculated, x * 45000, SDS-PAGE Papaver somniferum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.337 NMT3
-
 Papaver somniferum
2.1.1.337 RNMT
-
 Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.337 30
-
-
 Papaver somniferum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.337 0.028
-
 (S)-reticuline pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.047
-
 S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.053
-
 (R)-reticuline pH 7.0, 30°C Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.337 7 9 substrate (S)-reticuline Papaver somniferum
2.1.1.337 8.5
-
 substrate (R)-reticuline Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.337 S-adenosyl-L-methionine
-
 Papaver somniferum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.337 Papaver somniferum calculated
-
 5.9

General Information

EC Number General Information Comment Organism
2.1.1.337 physiological function suppression of the gene encoding reticuline N-methyltransferase results in a significant decrease in magnoflorine accumulation and a concomitant increase in corytuberine levels in roots Papaver somniferum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.337 0.28
-
 S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.62
-
 (R)-reticuline pH 7.0, 30°C Papaver somniferum
2.1.1.337 0.65
-
 (S)-reticuline pH 7.0, 30°C Papaver somniferum