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Literature summary extracted from

  • Yao, J.; Maxwell, J.B.; Rock, C.O.
    Resistance to AFN-1252 arises from missense mutations in Staphylococcus aureus enoyl-acyl carrier protein reductase (FabI) (2013), J. Biol. Chem., 288, 36261-36271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.10 expressed in Escherichia coli JP1111 cells Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.10 M99T the mutant is resistant toward inhibition by AFN-1252 Staphylococcus aureus
1.3.1.10 Y147F the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 Staphylococcus aureus
1.3.1.10 Y147H the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.10 AFN-1252
-
Staphylococcus aureus
1.3.1.10 triclosan
-
Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.10 Staphylococcus aureus
-
-
-
1.3.1.10 Staphylococcus aureus RN4220
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.10 Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.10 crotonyl-CoA + NADPH + H+
-
Staphylococcus aureus ?
-
?
1.3.1.10 crotonyl-CoA + NADPH + H+
-
Staphylococcus aureus RN4220 ?
-
?
1.3.1.10 crotonyl-[acyl-carrier protein] + NADPH + H+
-
Staphylococcus aureus ?
-
?
1.3.1.10 crotonyl-[acyl-carrier protein] + NADPH + H+
-
Staphylococcus aureus RN4220 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.10 enoyl-[acyl-carrier-protein] reductase
-
Staphylococcus aureus
1.3.1.10 FabI
-
Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.10 0.038
-
crotonyl-[acyl-carrier protein] mutant enzyme Y147F, pH and temperature not specified in the publication Staphylococcus aureus
1.3.1.10 0.038
-
crotonyl-[acyl-carrier protein] mutant enzyme Y147H, pH and temperature not specified in the publication Staphylococcus aureus
1.3.1.10 0.51
-
crotonyl-[acyl-carrier protein] wild type enzyme, pH and temperature not specified in the publication Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.10 NADH
-
Staphylococcus aureus
1.3.1.10 NADPH
-
Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.10 0.000004
-
AFN-1252 wild type enzyme, pH and temperature not specified in the publication Staphylococcus aureus
1.3.1.10 0.000069
-
AFN-1252 mutant enzyme M99T, pH and temperature not specified in the publication Staphylococcus aureus