EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.10 | expressed in Escherichia coli JP1111 cells | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.10 | M99T | the mutant is resistant toward inhibition by AFN-1252 | Staphylococcus aureus |
1.3.1.10 | Y147F | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
1.3.1.10 | Y147H | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.10 | AFN-1252 | - |
Staphylococcus aureus | |
1.3.1.10 | triclosan | - |
Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.10 | Staphylococcus aureus | - |
- |
- |
1.3.1.10 | Staphylococcus aureus RN4220 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.10 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.10 | crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
1.3.1.10 | crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? | |
1.3.1.10 | crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
1.3.1.10 | crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.10 | enoyl-[acyl-carrier-protein] reductase | - |
Staphylococcus aureus |
1.3.1.10 | FabI | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.10 | 0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147F, pH and temperature not specified in the publication | Staphylococcus aureus | |
1.3.1.10 | 0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147H, pH and temperature not specified in the publication | Staphylococcus aureus | |
1.3.1.10 | 0.51 | - |
crotonyl-[acyl-carrier protein] | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.10 | NADH | - |
Staphylococcus aureus | |
1.3.1.10 | NADPH | - |
Staphylococcus aureus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.10 | 0.000004 | - |
AFN-1252 | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus | |
1.3.1.10 | 0.000069 | - |
AFN-1252 | mutant enzyme M99T, pH and temperature not specified in the publication | Staphylococcus aureus |