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Literature summary extracted from
- Combined purification of actinomycin synthetase I and 3-hydroxyanthranilic acid 4-methyltransferase from Streptomyces antibioticus (1993), J. Biol. Chem., 268, 6831-6834.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.97 | 45000 | - |
x * 45000, SDS-PAGE | Streptomyces antibioticus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.7.97 | ATP + 3-hydroxy-4-methylanthranilate | Streptomyces antibioticus | - |
diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.7.97 | Streptomyces antibioticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.7.97 | DEAE-cellulose column chromatography, phenyl Sepharose column chromatography, QAE-Sepharose column chromatography, and Sephacryl gel filtration | Streptomyces antibioticus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.7.97 | mycelium | - |
Streptomyces antibioticus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.97 | 0.0008 | - |
crude enzyme, at pH 7.5 and 37°C | Streptomyces antibioticus |
| 2.7.7.97 | 0.795 | - |
after 958fold purification, at pH 7.5 and 37°C | Streptomyces antibioticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.7.97 | ATP + 3-hydroxy-4-methylanthranilate | - |
Streptomyces antibioticus | diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
| 2.7.7.97 | ATP + 3-hydroxyanthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
| 2.7.7.97 | ATP + 3-hydroxybenzoate | - |
Streptomyces antibioticus | ? | - |
? | |
| 2.7.7.97 | ATP + anthranilate | - |
Streptomyces antibioticus | ? | - |
? | |
| 2.7.7.97 | ATP + benzoate | - |
Streptomyces antibioticus | ? | - |
? | |
| 2.7.7.97 | ATP + glycine | low activity | Streptomyces antibioticus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.7.97 | ? | x * 45000, SDS-PAGE | Streptomyces antibioticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.7.97 | ACMSI | - |
Streptomyces antibioticus |
| 2.7.7.97 | actinomycin synthetase I | - |
Streptomyces antibioticus |
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