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Literature summary extracted from

  • Keller, U.; Schlumbohm, W.
    Purification and characterization of actinomycin synthetase I, a 4-methyl-3-hydroxyanthranilic acid-AMP ligase from Streptomyces chrysomallus (1992), J. Biol. Chem., 267, 11745-11752.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.97 0.0004
-
 3-hydroxy-4-methylanthranilate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 0.005
-
 3-hydroxy-4-methylbenzylate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 0.018
-
 3-Hydroxy-anthranilate at pH 8.0 and 28°C Streptomyces anulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.97 45000
-
 x * 45000, SDS-PAGE Streptomyces anulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.97 ATP + 3-hydroxy-4-methylanthranilate Streptomyces anulatus
-
 diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-4-methylanthranilate Streptomyces anulatus X2-18
-
 diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.97 Streptomyces anulatus
-
-
-
2.7.7.97 Streptomyces anulatus X2-18
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.97 polymin P precipitation, DEAE-cellulose column chromatography, ammonium sulfate precipitation, Aca-44 gel filtration, aminohexyl Sepharose column chromatography, Aca-54 gel filtration, and Mono Q column chromatography Streptomyces anulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.97 0.024
-
 crude extract, at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 0.38
-
 after 15.34fold purification, at pH 8.0 and 28°C Streptomyces anulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.97 ATP + 3-hydroxy-4-methylanthranilate
-
 Streptomyces anulatus diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-4-methylanthranilate
-
 Streptomyces anulatus X2-18 diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-4-methylbenzylate
-
 Streptomyces anulatus diphosphate + 3-hydroxy-4-methylbenzyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-4-methylbenzylate
-
 Streptomyces anulatus X2-18 diphosphate + 3-hydroxy-4-methylbenzyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-anthranilate
-
 Streptomyces anulatus diphosphate + 3-hydroxy-anthranilyl-adenylate
-
 ?
2.7.7.97 ATP + 3-hydroxy-anthranilate
-
 Streptomyces anulatus X2-18 diphosphate + 3-hydroxy-anthranilyl-adenylate
-
 ?
2.7.7.97 additional information the enzyme does not act as an acyl-coenzyme A ligase and has no activity with benzoic acid, 2-aminobenzoic acid, 3-aminobenzoic acid, 4-aminobenzoic acid, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 3-methylbenzoic acid, and 4-methylbenzoic acid Streptomyces anulatus ?
-
 ?
2.7.7.97 additional information the enzyme does not act as an acyl-coenzyme A ligase and has no activity with benzoic acid, 2-aminobenzoic acid, 3-aminobenzoic acid, 4-aminobenzoic acid, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 3-methylbenzoic acid, and 4-methylbenzoic acid Streptomyces anulatus X2-18 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.97 ? x * 45000, SDS-PAGE Streptomyces anulatus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.97 ACMS I
-
 Streptomyces anulatus
2.7.7.97 actinomycin synthetase I
-
 Streptomyces anulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.97 0.0032
-
 3-hydroxy-4-methylanthranilate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 0.005
-
 3-Hydroxy-anthranilate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 0.007
-
 3-hydroxy-4-methylbenzylate at pH 8.0 and 28°C Streptomyces anulatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.97 0.3
-
 3-Hydroxy-anthranilate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 1.1
-
 3-hydroxy-4-methylbenzylate at pH 8.0 and 28°C Streptomyces anulatus
2.7.7.97 7.8
-
 3-hydroxy-4-methylanthranilate at pH 8.0 and 28°C Streptomyces anulatus