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Literature summary extracted from

  • Zhang, Y.; Ning, F.; Li, X.; Teng, M.
    Structural insights into cofactor recognition of yeast mitochondria 3-oxoacyl-ACP reductase OAR1 (2013), IUBMB Life, 65, 154-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.100 apo-form complexed with NADPH, vapor diffusion method, using 10% (w/v) polyethylene glycol 4000, 10% (v/v) 2-propanol, 0.1 M HEPES pH 7.0 and 30% (v/v) jeffamine ED2001, 0.1 M HEPES pH 7.5 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.100 R14A inactive Saccharomyces cerevisiae
1.1.1.100 S36A the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
1.1.1.100 S40A the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.0205
-
 NADPH wild type enzyme, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 0.0264
-
 NADPH mutant enzyme S36A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 0.0571
-
 NADPH mutant enzyme S40A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 0.8158
-
 acetoacetyl-CoA wild type enzyme, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 0.8871
-
 acetoacetyl-CoA mutant enzyme S36A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 0.9913
-
 acetoacetyl-CoA mutant enzyme S40A, at pH 8.0 and 25°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.100 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ Saccharomyces cerevisiae
-
 a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Saccharomyces cerevisiae P35731
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100 Ni-NTA resin column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+
-
 Saccharomyces cerevisiae a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 homodimer x-ray crystallography Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 3-oxoacyl-(acyl-carrier-protein) reductase
-
 Saccharomyces cerevisiae
1.1.1.100 3-oxoacyl-ACP reductase
-
 Saccharomyces cerevisiae
1.1.1.100 FabG
-
 Saccharomyces cerevisiae
1.1.1.100 OAR1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.100 91.4
-
 NADPH mutant enzyme S40A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 91.4
-
 acetoacetyl-CoA mutant enzyme S40A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 108.2
-
 NADPH mutant enzyme S36A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 108.2
-
 acetoacetyl-CoA mutant enzyme S36A, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 117.5
-
 NADPH wild type enzyme, at pH 8.0 and 25°C Saccharomyces cerevisiae
1.1.1.100 117.5
-
 acetoacetyl-CoA wild type enzyme, at pH 8.0 and 25°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Saccharomyces cerevisiae