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Literature summary extracted from

  • Chen, L.; Yang, J.
    Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol (2008), Int. J. Mol. Sci., 9, 198-212.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.404 tetrachlorohydroquinone activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone Sphingobium chlorophenolicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.404 expression in Eshcerichia coli Sphingobium chlorophenolicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.404 homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN Sphingobium chlorophenolicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.404 Pentachlorophenol competitive, 55% loss of activity at 0.1 mM Sphingobium chlorophenolicum
1.1.1.404 tetrachlorohydroquinone activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone Sphingobium chlorophenolicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.404 Iron protein contains 1.7 mol of iron per mol Sphingobium chlorophenolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.404 additional information
-
 protein exists as trimer, dynamic light scattering Sphingobium chlorophenolicum
1.1.1.404 36000
-
-
 Sphingobium chlorophenolicum
1.1.1.404 36500
-
-
 Sphingobium chlorophenolicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.404 Sphingobium chlorophenolicum Q47914
-
-
1.1.1.404 Sphingobium chlorophenolicum ATCC 39723 Q47914
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.404 recombinant protein,. Purified enzyme is colorless Sphingobium chlorophenolicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.404 2.1
-
 pH 7.0, 25°C Sphingobium chlorophenolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.404 2,3,5,6-tetrachlorohydroquinone + NAD+
-
 Sphingobium chlorophenolicum 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
 ?
1.1.1.404 2,3,5,6-tetrachlorohydroquinone + NAD+
-
 Sphingobium chlorophenolicum ATCC 39723 2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.404 homotrimer 3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated Sphingobium chlorophenolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.404 7
-
-
 Sphingobium chlorophenolicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.404 5
-
 70% of maximum activity Sphingobium chlorophenolicum
1.1.1.404 8
-
 20% of maximum activity Sphingobium chlorophenolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.404 FMN the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster Sphingobium chlorophenolicum
1.1.1.404 [2Fe-2S]-center the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster Sphingobium chlorophenolicum