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Literature summary extracted from

  • Ruszkowski, M.; Nocek, B.; Forlani, G.; Dauter, Z.
    The structure of Medicago truncatula delta(1)-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants (2015), Front. Plant Sci., 6, 869.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.2 Cl- progressive stimulation by chloride ions from 20-200 mM, inhibitory above. At 100 mM, 1.8fold stimulation in presence of NADH Medicago truncatula

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 expression in Escherichia coli Medicago truncatula

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.2 crystal structures of unliganded P5CR decamer, and its complexes with the products NAD+, NADP+, and L-proline, to 1.7, 1.85, 1.95, and 2.1 A resolution, respectively Medicago truncatula

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 Cl- progressive stimulation from 20-200 mM, inhibitory above Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.02
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, pH 7.5, 35°C Medicago truncatula
1.5.1.2 0.039
-
 NADPH pH 7.5, 35°C Medicago truncatula
1.5.1.2 0.24
-
 1-pyrroline-5-carboxylate cosubstrate NADH, pH 7.5, 35°C Medicago truncatula
1.5.1.2 0.468
-
 NADH pH 7.5, 35°C Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Medicago truncatula G7KRM5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 leaf
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Medicago truncatula L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Medicago truncatula L-proline + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 More enzyme is a decamer of five dimers, crystallization data Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 MTR_7g090160
-
 Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 1.113
-
 NADPH pH 7.5, 35°C Medicago truncatula
1.5.1.2 2.972
-
 NADH pH 7.5, 35°C Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Medicago truncatula
1.5.1.2 NADPH
-
 Medicago truncatula

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.2 0.485
-
 cofactor NADH, pH 7.5, 35°C Medicago truncatula Cl-
1.5.1.2 0.704
-
 cofactor NADPH, pH 7.5, 35°C Medicago truncatula Cl-

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.2 6350
-
 NADH pH 7.5, 35°C Medicago truncatula
1.5.1.2 28500
-
 NADPH pH 7.5, 35°C Medicago truncatula